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Molecular technique

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  • Acta Crystallographica Section C: Structural Chemistry specializes in the rapid dissemination of high-quality detailed studies of novel and challenging crystal and molecular structures of interest in the fields of chemistry, biochemistry, mineralogy, pharmacology, physics and materials science. The unique checking, editing and publishing facilities of the journal ensure the highest standards of structural reliability and presentation, while providing for reports on studies involving special techniques or difficult crystalline materials.

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  • Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques.

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  • Mycoplasma suis (M. suis) belongs to the group of haemotrophic mycoplasmas and is known as the causative agent of infectious anaemia in pigs. In the last few years valuable insights into the mechanism of adhesion and invasion, shedding patterns and cell tropism of M. suis were gained by the use of new molecular techniques.

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  • Cats can be carriers of infected arthropods and be infected with several vector-borne pathogens (VBPs) but there is limited knowledge about their pathogenic role in cats. This study aimed to assess the prevalence of some feline vector-borne agents by molecular technique and to characterize the hematological findings associated with these infections in a cat population from Bangkok Thailand.

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  • Anaplasma and Ehrlichia species are tick-borne pathogens of both veterinary and public health importance. The current status of these pathogens, including emerging species such as Ehrlichia minasensis and Anaplasma platys, infecting cattle in Kenya, remain unclear, mainly because of limitation in the diagnostic techniques. Therefore, we investigated the Anaplasma and Ehrlichia species infecting dairy cattle in Nairobi, Kenya using molecular methods.

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  • Toxoplasma gondii is an important parasite that belongs to the phylum Apicomplexa, distributed globally, causing major health issues for a wide range of hosts, including humans, native and wild animals.

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  • Hybrid genome assembly has emerged as an important technique in bacterial genomics, but cost and labor requirements limit large-scale application. We present Ultraplexing, a method to improve per-sample sequencing cost and hands-on time of Nanopore sequencing for hybrid assembly by at least 50% compared to molecular barcoding while maintaining high assembly quality.

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  • Lecture ECE 6450 Microelectronic - Chapter 10: Vacuum Technology and plasmas. After studying this section will help you understand deposition techniques such as evaporation, sputtering, , plasma processing, chemical vapor deposition, molecular beam epitaxy, etc…, we must first have a grasp of the manner in which vacuum and gases flow. Thus, we will begin with a discussion of vacuum physics and vacuum techniques.

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  • The SDS–PAGE and ISSRPCR techniques were used to detect some biochemical and molecular markers associated with drought tolerance in six local and exotic genotypes of lentil (Sinai 1, Giza 9, L 9, L 16, L 20 and L 25) under rainfed conditions at El-sheikh Zowaid, North Sinai.

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  • Mosquitoes are one of the organisms subjected to frequent insecticide application due to their status as vectors that carry a wide range of life-threatening diseases. Turkey has climatic and other ecological features required for the breeding and living of 50 species from 8 genera. The members of the Culex pipiens complex are the dominant mosquitoes among them. In order to design more sustainable insecticide resistance management strategies, it is important to investigate the genetic structure of mosquito populations using molecular techniques.

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  • Knowledge of a predator’s diet is important for understanding its ecology and for predicting its influence on the dynamics of prey populations. We investigated the spatial distribution and diet composition of the leopard (Panthera pardus) in the northeastern Himalayan region of Pakistan. We used molecular scatology technique to identify scats of common leopard collected from the field. The leopard was recorded at 30 different surveyed sites with an elevational range between 757–1891 m a.s.l. Its diet comprised 17 prey species, including both wild and domestic prey.

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  • Polycarbazole/chitosan composite materials were synthesized electrochemically at various loadings of chitosan (Chi). Their electrochemical, structural, thermal, and morphological characterizations were investigated by cyclic voltammetry, chronoamperometry, electrochemical impedance spectroscopy, Fourier transform infrared spectroscopy, thermal gravimetry, and scanning electron microscopy. Further electrical conductivity was measured using a four-point probe technique.

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  • Lecture Bioinformatics introduction. After completing this section, you will understand knowledge about: Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with these molecules, on a large-scale.

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  • Extraction techniques in analytical science present the content: pre- and post-extraction considerations, classical approaches for aqueous extraction, solid phase extraction, solid phase microextraction, new developments in microextraction, classical approaches for solid–liquid extraction, pressurized fluid extraction, microwave-assisted extraction, matrix solid phase dispersion, supercritical fluid extraction, comparison of extraction methods,...

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  • An integrated approach to the study of taxa of the genus Hypericum occurring in Sicily is proposed. The results of morphological, biochemical, and molecular analyses are combined to better assess the relationships between the species investigated and test the suitability of DNA barcoding technique in the discrimination of these taxa. For the name Hypericum aegypticum subsp. webbii (Spach) N. Robson a lectotype is designated. For Hypericum triquetrifolium Turra a lectotype and a supporting epitype are designated. The presence of Hypericum perforatum L. subsp.

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  • Nonsteroidal antiinflammatory drugs (NSAIDs) are widely used for the treatment of pain and inflammation. Some undesirable effects are linked with NSAIDs such as the gastrointestinal tract (GIT) toxicity and cardiovascular disturbances. At present the preparation of a hybrid molecular technique is being used to produce new analgesic and antiinflammatory molecules. Attachment of NSAIDs with nitric oxide and hydrogen sulfide releasing molecules produced some gastroprotective agents with improved analgesic and antiinflammatory activities.

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  • A binding stability order predicting, molecular docking based fast technique was developed for host–guest complexes. Molecular descriptors were applied to ligand molecules to make the binding energy based docking scoring functions more efficient and reach the ±0.50 log K unit theoretical precision for predictions. The goal of this work was to model complexes of cucurbit[7]uril (CB7) as a host molecule with different local anesthetics and choline and phosphonium choline molecules.

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  • A series of 5-substituted-1,3,4-thiadiazole-based fluoroquinolone derivatives were designed as potential antibacterial and anticancer agents using a molecular hybridization approach. The target compounds 16–25 were synthesized by reacting the corresponding N-(5-substituted-1,3,4-thiadiazol-2-yl)-2-chloroacetamides with ciprofloxacin or norfloxacin. The purity and identity of the synthesized compounds were determined by the use of chromatographic and spectral techniques (NMR, IR, MS, etc.) besides elemental analysis.

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  • The nucleophilic substitution reactions of mono- and bis-spiro-2,2′ -dioxybiphenyl cyclotriphosphazenes (3 and 4) with cyclopropanemethylamine (5) and aniline (6) were performed in the presence of trimethylamine in THF. Five novel cyclopropanemethylamino- and anilino-substituted spiro-2,2′ -dioxybiphenyl cyclotriphosphazene derivatives (7–11) were obtained from these reactions. The molecular structures of the new cyclotriphosphazene derivatives (7– 11) were characterized by elemental analysis, MALDI-TOF MS, FT-IR, and NMR ( 31P and 1H) spectroscopies.

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  • Cancer is one of the main global health problems. In order to develop novel antitumor agents, we synthesized 3,4-dihydropyrimidine-2(1H)-one (DHPM) and 2,6-diaryl-substituted pyridine derivatives as potential antitumor structures and evaluated their cytotoxic effects against several cancer cell lines. An easy and convenient method is reported for the synthesis of these derivatives, employing cobalt ferrite (CoFe 2 O4 @SiO2 -SO3 H) magnetic nanoparticles under microwave irradiation and solvent-free conditions.

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