Phosphate rate

Mục tiêu của nghiên cứu này là xác định mật độ sạ và mức độ phân lân áp dụng cho lúa đạt năng suất và hiệu quả kinh tế cao. Thí nghiệm được thực hiện theo khối hoàn toàn ngẫu nhiên với 3 lặp lại và 2 nhân tố (3 mật độ sạ: 100, 160 và 220 kg/ha và 2 liều lượng lân: 0 và 50 kg P2O2/ha).

5p bautroibinhyen17 13-02-2017 46 2   Download

The involvement of the lysine residue present at the active site of Ehrlich ascites carcinoma (EAC) cell glyceraldehyde-3-phosphate dehydrogenase (Gra3P DH) was investigated by using the lysine specific reagents trinitrobenzenesulfonic acid (TNBS) and pyridoxal phosphate (PP). Both TNBS and PP inactivated EAC cell Gra3P DH with pseudo-first-order kinetics with the rate dependent on modifier concentration. Kinetic analysis, including a Tsou plot, indicated that both TNBS and PP apparently react with one lysine residue per enzyme molecule.

8p system191 01-06-2013 37 3   Download

Cloning and over-expression of human glucose 6-phosphate dehydrogenase (Glc6P dehydrogenase) has for the first time allowed a detailed kinetic study of a preparation that is genetically homogeneous and in which all the protein molecules are of identical age. The steady-state kinetics of the recombinant enzyme, studied by fluorimetric initial-rate measurements, gave converging linear Lineweaver–Burk plots as expected for a ternary-complex mechanism.

8p research12 01-06-2013 59 4   Download

Heterologous expression of the putative open reading frame MJ0303 ofMethanococcus jannaschiiprovided a recombin-ant protein catalysing the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, by condensation of 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione and 3,4-dihydroxy-2-butanone 4-phosphate. Steady state kinetic analysis at 37
C and pH 7.0 indicated a catalytic rate of 11 nmolÆmg )1 Æmin )1 ;Kmvalues for 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione and 3,4-dihydroxybutanone 4-phosphate were 12.5 and 52lM, respectively....

8p tumor12 20-04-2013 37 3   Download

To shed light on the mechanism of isotopic exchange of a-protons in amino acids catalyzed by pyridoxal phosphate (PLP)-dependent enzymes, we studied the kinetics of quinonoid intermediate formation for the reactions of tyrosine phenol-lyase with L-phenylalanine, L-methionine, and theira-deuterated analogues inD2O, andwe compared the results with the rates of the isotopic exchange under the same conditions.Wehave found that, in theL-phenylalanine reaction, the internal returnof thea-proton is operative, and allowing for its effect, the exchange rate is accounted for satisfactorily....

7p awards 05-04-2013 50 4   Download

Using 0.4Mimidazole citrate buffer (pH 7.5) containing 0.1 mML-cysteine, homodimeric starch phosphorylase from Corynebacterium calluane(CcStP) was dissociated into native-like folded subunits concomitant with release of pyridoxal 5¢-phosphate and loss of activity. The inactivation rate of CcStP under resolution conditions at 30
Cwas, respectively, four- and threefold reduced in two mutants, Arg234fiAla and Arg242fiAla, previously shown to cause thermostabilization of CcStP [Griessler, R., Schwarz, A., Mucha, J.&Nidetzky, B. ...

11p awards 05-04-2013 41 5   Download

TheispC gene of Arabidopsis thalianawas expressed in pseudomature form without the putative plastid-targeting sequence in a recombinant Escherichia colistrain. The recombinant protein was purified by affinity chromatography and was shown to catalyze the formation of 2C-methyl-d-erythritol 4-phosphate from 1-deoxy-d-xylulose 5-phosphate at a rate of 5.6lmolÆmin )1 Æmg )1 (kcat 4.4 s )1 ). The Michaelis constants for 1-deoxy-d-xylulose 5-phosphate and the cosubstrate NADPH are 132 and 30lm, respectively. ...

13p inspiron33 25-03-2013 36 5   Download

Clostridial glutamate dehydrogenase mutants, designed to accommodate the 2¢-phosphate of disfavoured NADPH, showed the expected large speci-ficity shifts with NAD(P)H. Puzzlingly, similar assays with oxidized cofac-tors initially revealed little improvement with NADP + , although rates with NAD + were markedly diminished.

9p cosis54 05-01-2013 49 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Salivary flow rate, pH, and concentrations of calcium, phosphate, and sIgA in Brazilian pregnant and non-pregnant women

5p panasonic05 22-12-2011 56 4   Download

Tách Protein sử dụng cột GFC Analytical Conditions Column : Asahipak GFA-50 Mobile phase : 0.1 M sodium phosphate 0.1 M NaCl (pH=7.0) Flow rate : 0.5 mL/min Temperature : ambient Detector : UV-280 nm Injection volume : 10 uL Peaks 1. glutamate dehydrogenase 2. lactate dehydrogenase 3. enolase 4. adenylate kinase 5. cytochrome C

10p zues05 23-06-2011 117 22   Download