Transferases

Mục tiêu của đề tài là đánh giá sự tích tụ KLN (Cu, Pb, Zn và Cd) trong mang, gan, thận của cá chép và cá rô phi theo mùa và theo mặt cắt trong khu vực nghiên cứu; phân tích sự biến động của nồng độ protein và hoạt tính GST trong cùng mẫu cá phân tích kim loại nặng; bước đầu xác định tương quan giữa nồng độ kim loại nặng tích tụ với nồng độ protein và hoạt tính GST trong cùng mẫu cá phân tích.

82p closefriend03 13-10-2021 28 7   Download

Đề tài nghiên cứu nhằm 3 mục tiêu: biến nạp thành công gen Isopentenyl transferase vào một số dòng ngô chọn lọc, kiểm tra sự có mặt của gen ipt bằng kỹ thuật sinh học phân tử, bước đầu đánh giá khả năng chịu hạn ở cây con thế hệ T1.

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Luận văn tập trung nghiên cứu tạo ra dòng mô sẹo chuyển gen, bước đầu đánh giá sự hiện diện và biểu hiện của các gen chuyển bằng thử nghiệm định tính (thử GUS) và phương pháp sinh học phân tử (PCR). Mời các bạn cùng tham khảo nội dung chi tiết.

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Khóa luận được nghiên cứu với mục tiêu nhằm đặc điểm lâm sàng và cận lâm sàng ở bệnh nhân gút tăng gammaglutamyl transferase tại Bệnh viện E từ tháng 06/2017 đến tháng 03/2018. Phân tích mối liên quan giữa gamma-glutamyl transferase tới một số chỉ số sinh hóa và lâm sàng.

56p chuheodethuong25 12-07-2021 27 8   Download

Luận văn "Bước đầu nghiên cứu chuyển gen ipt (isopentenyl transferase) vào mô sẹo sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.)" được đặt ra với mục đích tạo được mô sẹo sâm Ngọc Linh mang gen ipt bằng kỹ thuật biến nạp gen phục vụ cho mục đích nghiên cứu dài hạn về ảnh hưởng của gen chuyển ipt đến khả năng tăng sinh tổng hợp các chất thứ cấp. Mời các bạn cùng tham khảo.

108p dangthingocthuy96 09-01-2017 71 18   Download

Previously, we demonstrated apoptotic cell death in the chorion laeve trophoblast layer of human fetal membrane tissues during the late stages of pregnancy, the progression of apoptosis during incubation in vitro, and its suppression by a low concentration of glucocorticoid hormones.

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Two genes (CM-AAT1 and CM-AAT2) with strong sequence homology (87% identity at the protein level) putatively involved in the formation of aroma volatile esters have been isolated from Charentais melon fruit. They belong to a large and highly divergent family of multifunctional plant acyl-transferases and show at most 21% identity to the only other fruit acyl-transferase characterized so far in strawberry. RT-PCR studies indicated that both genes were specifically expressed in fruit at increasing rates in the early and mid phases of ripening.

8p research12 01-06-2013 49 4   Download

Binding of lamin B receptor (LBR) to chromatin was studied by means of an in vitro assay system involving recombinant fragments of human LBR and Xenopus sperm chromatin. Glutathione-S-transferase (GST)-fused proteins including LBR fragments containing the N-terminal region (residues 1–53) and arginine-serine repeat-containing region (residues 54–89) bound to chromatin. The binding of GST-fusion proteins incorporating the N-terminal and arginine-serine repeat-containing regions to chromatin was suppressed by mild trypsinization of the chromatin and by pretreatment with a DNA solution....

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Antisense oligodeoxynucleotides (AS-ODNs) are frequently used for the down-regulation of protein expression.Because the majority of potential antisense sequences lacks effect-iveness, fast screening methods for the selection of effective AS-ODNs are needed.We describe a new cellular screening assay for the evaluationof the potency and specificityof new antisense sequences.

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Propionate CoA-transferase fromClostridium propionicum hasbeenpuri®edand thegene encoding the enzymehasbeen cloned and sequenced. The enzyme was rapidly and irre-versibly inactivated by sodium borohydride or hydroxyl-amine in the presence of propionyl-CoA. The reduction of the thiol ester between a catalytic site glutamate and CoA with borohydride and the cleavage by hydroxylamine were used to introduce a site-speci®c label, which was followed by MALDI-TOF-MS. This allowed the identi®cation of glutamate 324 at the active site. ...

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b1,4-N-Acetylglucosaminyltransferase III (GnT-III) plays a regulatory role in the biosynthesis of N-glycans, and it has been suggested that its product, a bisecting GlcNAc, is involved in a variety of biological events as well as in regu-lating the biosynthesis of the oligosaccharides. In this study, it was found, on the basis of sequence homology, that GnT-III contains a small region that is signi®cantly homologous to both snail b1,4GlcNAc transferase andb1,4Gal trans-ferase-1.

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This paper deals with the purification and the partial char-acterization of glutathione S-transferase (GST) isoforms from the clam Ruditapes decussatus. For the first step of purification, two affinity columns, reduced glutathione (GSH)–agarose and S-hexyl GSH–agarose, were mounted in series. Four affinity fractionswere thus recovered. Further purification was performed using anion exchange chroma-tography. Seven fractions,whichpresent aGSTactivitywith 1-chloro-2,4-dinitrobenzene (CDNB) as substrate, were collected and analyzed by RP-HPLC....

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Biosynthesis ofmucin-typeO-glycans is initiated by a family of UDP-GalNAc:polypeptide N-acetylgalactosaminyl-transferases, which contain several conserved cysteine resi-dues among the isozymes. We found that a cysteine-specific reagent, p-chloromercuriphenylsulfonic acid (PCMPS), irreversibly inhibited one of the isozymes (GalNAc-T1). Presence of either UDP-GalNAc or UDP during PCMPS treatment protected GalNAc-T1 from inactivation, to the same extent.

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O-Glycosylation of three consecutive Thr residues in a fluorescein-conjugated peptide PTTTPLK)which mimics a portion of mucin 2)by four isozymes of UDP-N-ace-tylgalactosaminyltransferases (pp-GalNAc-T1, T2, T3, or T4) was investigated. Partially glycosylated versions of this peptide, PT*TTPLK, PTTT*PLK, PT*TT*PLK, PTT*T*PLK, PT*
TTPLK, andPTTT*
PLK(*,N-acetyl-galactosamine;
, galactose), were also tested.

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GlutathioneS-transferases (EC 2.5.1.18) (GSTs), are a family of multifunctional enzymes present in all living organisms whose main function is the detoxification of electrophilic compounds. GSTs are considered the most prominent detoxifying class II enzymes in helminths. We describehere the characterizationof novel dehydroascorbate reductase and thiol transferase activities that reside in the human parasite Schistosoma mansoni GSTx.

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Thirteen glucose analogues bearing electrophilic groups were synthesized (five of themfor the first time) and screened as inhibitors of the glucose transporter (EII Glc )of the Escherichia coli phosphoenolpyruvate–sugar phospho-transferase system (PTS). 2¢,3¢-Epoxypropyl b-D-glucopyr-anoside (3a) is an inhibitor and also a pseudosubstrate. Five analogues are inhibitorsofnonvectorialGlcphosphorylation by EII Glc but not pseudosubstrates.

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Rubber transferase, acis-prenyltransferase, catalyzes the addition of thousands of isopentenyl diphosphate (IPP) molecules toanallylic diphosphate initiator, suchas farnesyl diphosphate (FPP,1), in the presence of a divalent metal cofactor. In an effort to characterize the catalytic site of rubber transferase, the effects of two types of protein farnesyltransferase inhibitors, several chaetomellic acid A analogs (2,4–7)anda-hydroxyfarnesylphosphonic acid (3), on the ability of rubber transferase to add IPP to the allylic diphosphate initiator were determined. ...

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The a-amylase family (glycoside hydrolase family 13; GH 13) contains enzymes with approximately 30 specifi-cities. Six types of enzyme from the family can possess a C-terminal starch-binding domain (SBD): a-amylase, maltotetraohydrolase, maltopentaohydrolase, maltogenic a-amylase, acarviose transferase, and cyclodextrin glu-canotransferase (CGTase). Such enzymes are multidomain proteins and those that contain an SBD consist of four or five domains, the former enzymes being mainly hydrolases and the latter mainly transglycosidases. ...

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Platelet-activating factor (PAF) is one of the most potent inflammatory mediators. It is biosynthesized by either the de novobiosynthesis of glyceryl ether lipids or by remodeling ofmembranephospholipids. PAFis synthesizedandcatabo-lizedbyvarious renal cells andtissues andexerts awide range of biological activities on renal tissue suggesting a potential role during renal injury.

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High-resolution crystal structures of large ribosomal subunits from Deinococcus radioduranscomplexed with tRNA-mimics indicate that precise substrate positioning, mandatory for efficient protein biosynthesis with no further conformational rearrangements, is governed by remote interactions of the tRNA helical features.

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