Báo cáo khoa học: Donor substrate regulation of transketolase
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The influence of substrates on the interaction of apotrans-ketolase with thiamin diphosphate was investigated in the presence of magnesium ions. It was shown that the donor substrates, but not the acceptor substrates, enhance the affinity of the coenzyme either to only one active center of transketolase or to both active centers, but to different degrees in each, resulting in a negative cooperativity for coenzyme binding. In the absence of donor substrate, neg-ative cooperativity is not observed. ...
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