Báo cáo khoa học: Growth-associated protein of 43 kDa (GAP-43) is cleaved nonprocessively by the 20S proteasome
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Purified, nonubiquitinated growth-associated protein of 43 kDa (GAP-43) was attacked by purified reticulocyte 20S proteasomebutnotby the26Sproteasome.Cleavageyielded 12 N-terminally labelled GAP-43 fragments that could be resolved by SDS/PAGE. Inhibitor experiments suggested that proteasomeb1 activity yielded the resolved bands and that proteasome b5 activity generated nonresolvable frag-ments. Processive degradation, yielding only nonresolvable fragments, therefore did not occur.
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