
Báo cáo khoa học: Measuring enzyme activities under standardized in vivo-like conditions for systems biology
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Realistic quantitative models require data from many laboratories. There-fore, standardization of experimental systems and assay conditions is crucial. Moreover, standards should be representative of thein vivoconditions. How-ever, most often, enzyme–kinetic parameters are measured under assay con-ditions that yield the maximum activity of each enzyme.
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