Báo cáo khoa học: The transmembrane domain of subunitbof theEscherichia coli F1FOATP synthase is sufficient for H + -translocating activity together with subunitsaandc

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:7

Thêm vào BST

Báo xấu

47
lượt xem 3
download

Download Vui lòng tải xuống để xem tài liệu đầy đủ

Subunitbis indispensable for the formation of a functional H + -translocating FOcomplex both in vivo andin vitro. Whereas the very C-terminus of subunitbinteracts with F1 and plays a crucial role in enzyme assembly, the C-terminal region is also considered to be necessary for proper recon-stitution of FOinto liposomes. Here, we show that a syn-thetic peptide, residues 1–34 of subunitb(b1)34 ) [Dmitriev, O., Jones, P.C., Jiang,W. & Fillingame, R.H. (1999)J. Biol. Chem.274, 15598–15604], corresponding to the membrane domain of subunitbwas sufficient in forming an active FO complex when coreconstitutedwith purifiedacsubcomplex....

Chủ đề: