Báo cáo Y học: Reconstitution of Fo of the sodium ion translocating ATP synthase of Propionigenium modestum from its heterologously expressed and purified subunits
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TheatpBandatpF genes ofPropionigenium modestumwere cloned as His-tag fusion constructs and expressed in Escherichia coli.Both recombinant subunits a and b were purified via Ni 2+ chelate affinitychromatography. A func-tionallyactive Focomplex was reassembledin vitro from subunits a, band c, and incorporated into liposomes.
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