Evolution of structure

This thesis consists of seven chapters. Chapter 1 provides a brief summary of the background of the study in addition to a discussion of the importance of safety, the evolution of aviation safety, an overview of the research context, the research objectives and questions, the significance of the research, and the structure of the thesis. Chapter 2 discusses military safety and in particular, the Indonesian military. Chapter 3 presents a literature review of culture in general and safety culture in particular, and the literature referenced to support the research questions.

244p runthenight04 02-02-2023 6 2   Download

The thesis "Performance measurement evolution and accountability in Indonesian regional governments" explores the development and implementation of a performance measurement system in Indonesian regional governments. A field study approach is employed in which publicly available and internal documents, together with semi-structured interviews, are used as a comprehensive source of evidence.

181p runthenight04 02-02-2023 11 5   Download

The study is expected to reach the following objectives: To study how idioms are organized structurally and semantically in English and Vietnamese; to establish the similarities and differences between English and Vietnamese idioms in terms of structural and semantic components; to provide main factors lying behind the similarities and differences between English and Vietnamese idioms.

196p badbuddy09 05-04-2022 25 6   Download

Structures of thesis: Chapter 1 - Preliminaries; Chapter 2 - Discrete data assimilation for the three-dimentional Leray-α model; Chapter 3 - Discrete data assimilation for the three-dimentional NavierStokes-α model; Chapter 4 - Abridged continuous data assimilation for the three-dimentional simplified Bardina model; Chapter 5 - Abridged data assimilation for the three-dimentional modified Leray-α.

26p tunelove 10-06-2021 21 3   Download

The case of homologous monomeric c-type and oligomeric b-type crystallins has been described and analyzed in evolutionary terms. Data and hypotheses from molecular genetics and structural investigations converge and suggest a novel three-phase model for the evolutionary history of crystallin-type proteins. In the divergent cascades of monomeric and oligomeric crystallins, a pivotal role was played by alterations in the gene segments encoding the C-terminal extensions and the intermotif or interdomain linker peptides.

9p system191 01-06-2013 41 4   Download

Cytochrome c is widely distributed in bacterial species, from mesophiles to thermophiles, and is one of the best-characterized redox proteins in terms of biogenesis, folding, structure, function, and evolution. Experimental molecular biology techniques (gene cloning and expression) have become applicable to cytochrome c, enabling its engineering and manipulation. Heterologous expression systems for cytochromes c in bacteria, for use in mutagenesis studies, have been established by extensive investigation of the biological process by which the functional structure is formed. ...

7p research12 01-06-2013 32 3   Download

The citric acid or tricarboxylic acid cycle is a central element of higher-plant carbon metabolism which provides, among other things, electrons for oxidative phosphorylation in the inner mitochondrial membrane, intermediates for aminoacid biosynthesis, and oxaloacetate for gluconeogenesis from succinate derived from fatty acids via the glyoxylate cycle in glyoxysomes. The tricarboxylic acid cycle is a typical mitochondrial pathway and is widespread among a-proteobacteria, the group of eubacteria as de®ned under rRNA systematics from which mitochondria arose. ...

16p research12 01-06-2013 41 3   Download

Murine double minute 2 (MDM2) protein exhibits many diverse biochemi-cal functions on the tumour suppressor protein p53, including transcrip-tional suppression and E3 ubiquitin ligase activity. However, more recent data have shown that MDM2 can exhibit ATP-dependent molecular chap-erone activity and directly mediate folding of the p53 tetramer. Analysing the ATP-dependent function of MDM2 will provide novel insights into the evolution and function of the protein.

12p research12 29-04-2013 61 4   Download

Acomprehensive, structural and functional, insilicoanalysis of the medium-chain dehydrogenase/reductase (MDR) superfamily, including 583 proteins, was carried out by use of extensive database mining and theBLASTPprogram in an iterative manner to identify all known members of the superfamily. Based on phylogenetic, sequence, and func-tional similarities, the protein members of the MDR super-family were classified into three different taxonomic categories: (a) subfamilies, consisting of a closed group containing a set of ideally orthologous proteins that perform the same function;...

26p tumor12 20-04-2013 35 5   Download

Hemocyanins are large oligomeric copper-containing pro-teins that serve for the transport of oxygen in many arth-ropod species. While studied in detail in the Chelicerata and Crustacea, hemocyanins had long been considered unnec-essary in the Myriapoda. Here we report the complete molecular structure of the hemocyanin from the common house centipedeScutigera coleoptrata(Myriapoda: Chilo-poda), as deduced from 2D-gel electrophoresis, MALDI-TOF mass spectrometry, protein and cDNA sequencing, and homology modeling....

9p fptmusic 16-04-2013 36 3   Download

TheDrosophila melanogasterdeoxyribonucleoside kinase (Dm-dNK) double mutant N45D⁄N64D was identified during a previous directed evolution study. This mutant enzyme had a decreased activity towards the natural substrates and decreased feedback inhibition with dTTP, whereas the activ-ity with 3¢-modified nucleoside analogs like 3¢-azidothymidine (AZT) was nearly unchanged.

10p fptmusic 11-04-2013 31 3   Download

The (ba)8-barrel is the most common enzyme fold and it is capable of cata-lyzing an enormous diversity of reactions. It follows that this scaffold should be an ideal starting point for engineering novel enzymes by directed evolution. However, experiments to date have utilized in vivo screens or selections and the compatibility of (ba)8-barrels with in vitro selection methods remains largely untested.

14p fptmusic 11-04-2013 41 3   Download

Thebandccrystallins are evolutionarily related families of proteins that make up a large part of the refractive structure of the vertebrate eye lens. Each family has a distinctive gene structure that reflects a history of succes-sive gene duplications. A survey ofc-crystallins expressed in mammal, rep-tile, bird and fish species (particularly in the zebrafish, Danio rerio) has led to the discovery of cN-crystallin, an evolutionary bridge between theband cfamilies.

16p awards 06-04-2013 39 2   Download

Higher plant chloroplasts possess at least four different pathways for pro-tein translocation across and protein integration into the thylakoid mem-branes. It is of interest with respect to plastid evolution, which pathways have been retained as a relic from the cyanobacterial ancestor (‘conserva-tive sorting’), which ones have been kept but modified, and which ones were developed at the organelle stage, i.e. are eukaryotic achievements as (largely) the Toc and Tic translocons for envelope import of cytosolic pre-cursor proteins. ...

12p awards 05-04-2013 36 4   Download

We purified pancreatic deoxyribonuclease I (DNase I) from the shark Heterodontus japonicususing three-step column chromatography. Although its enzymatic properties resem-bled those of other vertebrateDNases I, sharkDNase I was unique inbeingabasicprotein.Full-lengthcDNAs encoding theDNases I of two shark species,H. japonicusandTriakis scyllia, were constructed from their total pancreatic RNAs using RACE.

8p awards 05-04-2013 47 4   Download

The tissue-specific transcription factorsHNF1aandHNF1b are closely related homeodomain proteins conserved in vertebrate evolution. Heterozygous mutations in human HNF1b 1 but not inHNF1agenes are associated with kidney malformations. Overexpression of HNF1b in Xenopus embryos leads to defective pronephros development, while HNF1ahas no effect. We have defined the regions respon-sible for this functional difference between HNF1band HNF1ain transfected HeLa cells as well as in injected Xenopusembryos. ...

14p awards 05-04-2013 44 3   Download

L-Arabinitol 4-dehydrogenase (Lad1) of the cellulolytic and hemicellulolytic fungusHypocrea jecorina(anamorph: Trichoderma reesei) has been implicated in the catabolism ofL-arabinose, and genetic evidence also shows that it is involved in the catabolism of D-xylose in xylitol dehydrogenase (xdh1) mutants and of D-galactose in galactokinase (gal1) mutants of H. jecorina.In order to identify the substrate specificity of Lad1, we have recombinantly produced the enzyme inEscherichia coliand purified it to physical homogeneity....

9p dell39 03-04-2013 31 3   Download

We recently reported the unprecedented occurrence of a hemoglobin gene (glob1) in the fruitfly Drosophila melanogaster. Here we investigate the structure and evolution of the glob1gene in otherDrosophilaspecies. We cloned and sequencedglob1genes and cDNA fromD. pseudoobscuraand D. virilis, and identified the glob1gene sequences ofD. simulans, D. yak-uba, D. erecta, D. ananassae, D. mojavensisandD. grimshawiin the data-bases.

13p dell39 27-03-2013 39 4   Download

Patterns of protein interactions are organized around complex heterogene-ous networks. Their architecture has been suggested to be of relevance in understanding the interactome and its functional organization, which per-vades cellular robustness. Transcription factors are particularly relevant in this context, given their central role in gene regulation.

12p dell39 27-03-2013 39 3   Download

Crustacean hyperglycemic hormone (CHH) and vitellogenesis-inhibiting hormone (VIH), produced by the X organ–sinus gland neurosecretory com-plex, belong to a peptide group referred to as the CHH family, which is widely distributed in arthropods. In this study, genetic variants and post-translationally modified isoforms of CHH and VIH were characterized in the European lobster Homarus gammarus.

10p inspiron33 26-03-2013 51 4   Download