Genomic DNA

Hypertrophic cardiomyopathy (HCM) is a common genetic cardiovascular disease and a major cause of sudden death. It is also involved in increasing morbidity and mortality of various cardiovascular diseases. Genetic factors have been found to be important in determining the phenotypic manifestation of cardiac hypertrophy. However, only 50–60% of HCM patients have been identified as having pathogenic variants in known genes, suggesting that more studies are needed to find more disease genes.

15p xuanphongdacy06 18-09-2024 4 1   Download

Epigenetic defects (gain or loss of DNA methylation) of the human ICR1 11p15 domain result in two opposite foetal growth disorders (BWS and SRS) depending on which parental allele is affected. A few deletions within ICR1 have been reported in familial BWS cases with ICR1 gain of methylation, however the mechanism(s) of the DNA methylation defects at ICR1 remains largely unknown in most BWS and SRS patients.

83p runthenight07 01-03-2023 9 3   Download

Công nghệ chỉnh sửa genome đã mở ra một kỷ nguyên mới cho kỹ thuật thao tác trên vật liệu di truyền DNA. Kỹ thuật này được phát triển từ đầu những năm 1990, sau đó nhanh chóng trở thành công nghệ đột phá, đặc biệt trong chọn tạo giống cây trồng. Chỉnh sửa genome được ghi nhận là “phương pháp của năm” bởi tạp chí Nature Method, “bước đột phá” năm 2012, 2013 và 2015 bởi tạp chí Science.

4p viirenerosenfeld 18-05-2022 29 3   Download

Double-strand breaks (DSBs) result from the attack of both DNA strands by multiple sources, including radiation and chemicals. DSBs can cause the abnormal chromosomal rearrangements associated with cancer. Recent techniques allow the genome-wide mapping of DSBs at high resolution, enabling the comprehensive study of their origins.

14p vimichaelfaraday 25-03-2022 17 1   Download

Glioma stem cells (GSCs) are a subpopulation of stem-like cells that contribute to glioblastoma (GBM) aggressiveness, recurrence, and resistance to radiation and chemotherapy. Therapeutically targeting the GSC population may improve patient survival, but unique vulnerabilities need to be identified.

25p vimichaelfaraday 25-03-2022 24 1   Download

Early life exposure to adverse environments affects cardiovascular and metabolic systems in the offspring. These programmed effects are transmissible to a second generation through both male and female lines, suggesting germline transmission. We have previously shown that prenatal overexposure to the synthetic glucocorticoid dexamethasone (Dex) in rats reduces birth weight in the first generation (F1), a phenotype which is transmitted to a second generation (F2), particularly through the male line.

15p vimichaelfaraday 25-03-2022 16 2   Download

Recent sequencing technologies enable joint quantification of promoters and their enhancer regions, allowing inference of enhancer–promoter links. We show that current enhancer–promoter inference methods produce a high rate of false positive links. We introduce FOCS, a new inference method, and by benchmarking against ChIAPET, HiChIP, and eQTL data show that it results in lower false discovery rates and at the same time higher inference power.

14p vimichaelfaraday 25-03-2022 16 2   Download

DNA repair deficiencies are characteristic of cancer and homologous recombination deficiency (HRD) is the most common. HRD sensitizes tumour cells to PARP inhibitors so it is important to understand the landscape of HRD across diferent solid tumour types. Methods: Germline and somatic BRCA mu

13p vielonmusk 21-01-2022 17 2   Download

Circulating cell-free DNA (cfDNA) in the plasma of cancer patients contains cell-free tumour DNA (ctDNA) derived from tumour cells and it has been widely recognized as a non-invasive source of tumour DNA for diagnosis and prognosis of cancer.

13p vielonmusk 21-01-2022 20 0   Download

In this study, we evaluated the reliability of genomic DNAs and cloned recombinant plasmids as standard controls for absolute real-time PCR assay. The associated standard curves were constructed and used for the quantification of Lactobacillus acidophilus probiotics.

4p mudbound 10-12-2021 32 2   Download

The results showed that the RPA reaction, without requiring complex thermal cycles, was well-performed in the optimal conditions of 39°C within only 25 minutes. The limit of detection was identified as 310 fg of L. monocytogenes genomic DNA, which was 1000-fold more sensitive than the conventional PCR. RPA also succeeded to directly detect L. monocytogenes cells at a concentration as low as 2.5 × 101 Colony Forming Unit (CFU)/mL in pure cultures. In addition, RPA could accurately detect L. monocytogenes at 2.5 × 102 CFU/mL in milk without sample extraction or processing.

5p mudbound 10-12-2021 17 2   Download

The results showed that the designed primers were specific to only Foc isolates from race 1 and tropical race 4 (TR4). The detection efficiency of the designed primers was compared to other published primers through optimized SYBR green-based real-time PCR assay and nested PCR. The new primers could detect the Foc genomic DNA at a minimum of 5 pg and target pathogen in a symptomless banana sucker. The specificity and sensitivity of the new primers were comparable to the published real-time PCR primers and the nested PCR assay.

5p mudbound 10-12-2021 19 2   Download

Autophagy and DNA repair are two essential biological mechanisms that maintain cellular homeostasis. Impairment of these mechanisms was associated with several pathologies such as premature aging, neurodegenerative diseases, and cancer. Intrinsic or extrinsic stress stimuli (e.g., reactive oxygen species or ionizing radiation) cause DNA damage. As a biological stress response, autophagy is activated following insults that threaten DNA integrity.

18p thiencuuchu 27-11-2021 11 1   Download

Bài giảng Sinh học phân tử 1: Chương 3 Gen và genome, cung cấp cho người học những kiến thức như: Cấu trúc 1 gene ở prokaryote; Cấu trúc gen ở sinh vật Eukaryote; Chức năng của gene phân tử; Genome ty thể;... Mời các bạn cùng tham khảo!

52p caphesuadathemmatong 25-11-2021 31 3   Download

Bài viết trình bày phân tích trình tự đoạn gen COI phân lập từ mẫu cá nheo Thái Nguyên. Đoạn gen COI được phân lập từ DNA genome mẫu cá nheo bằng kỹ thuật PCR. Sau đó, sản phẩm PCR được tinh sạch và xác định trình tự, đoạn gen COI thu được có chiều dài 616 bp.

6p vidaegu2711 09-08-2021 20 3   Download

Đồ án tốt nghiệp này được thực hiện với mục tiêu nhằm ly trích và thu nhận DNA genomic từ mẫu vi khuẩn đã phân lập trong quá trình trồng ủ rơm; xác định nhóm vi khuẩn bằng phương pháp PCR của đoạn gene 16S rNDA dựa trên cặp mồi 27F và 1492R. Trình tự cặp mồi 27F 5‘-GA GTT TGA TCM TGG CTC AG-3‘, 1492R 5'-GG CTA CCT TGT TAC GAC TT-3'... Mời các bạn cùng tham khảo.

95p zhangyan 13-07-2021 35 6   Download

Bệnh khảm lá sắn (Cassava mosaic disease, CMD), do vi-rút thuộc họ Geminiviridae gây ra, là một trong 10 bệnh gây thiệt hại mùa màng lớn nhất trên thế giới. Trong nghiên cứu này, các mẫu lá sắn với bệnh tích khảm đặc trưng được thu thập tại Tây Ninh, Ninh Thuận và Củ Chi. Kết quả PCR xác định các mẫu dương tính với Sri Lanka cassava mosaic virus (SLCMV).

5p vitexas2711 02-11-2020 34 3   Download

To investigate biological roles of Myosin class I in the morphology of M. circinelloides and identify good candidates for further analyses of Mucor pathogenesis, we are going to inhibit their expression using RNAi technique. We named the two myosin class I like genes as M1L1 (ID 140500) and M1L2 (ID 116620). In the present study, we report the results of generation of RNAi plasmid to inhibit the expression of M1L1 gene.

6p koxih_kothogmih5 04-09-2020 40 3   Download

In this study, relative potential of 24 candidate loci (Dong et al, 2012) from plastid genome were validated on set of 231 diverse rice genotypes, for selection of suitable barcoding loci for DNA barcoding in rice.

11p cothumenhmong7 09-09-2020 16 3   Download

Foxtail millet (Setaria italica L. P. Beauv) has been considered as a tractable model crop in recent years due to its short growing cycle, lower amount of repetitive DNA, inbreeding nature, small diploid genome, and outstanding abiotic stress-tolerance characteristics.

19p vishikamaru2711 29-04-2020 10 0   Download