Loss of knowledge

Loss of knowledge is a critical factor for organizations. This paper approaches the topic of a combined research of knowledge management and succession planning.

12p guineverehuynh 22-06-2020 5 1   Download

Department of Medical Chemistry, Kochi Medical School, Nankoku, Japan; 2Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan; 3Department of Anatomy and Cell Biology, Kochi Medical School, Nankoku, Japan We previously generated mice lacking aromatase activity by targeted disruption of Cyp19 (ArKO mice), and reported phenotypes of the female mice, showing hemorrhage formation and follicular depletion in the ovary, diminution in uterine size, and bone loss...

9p research12 01-06-2013 44 5   Download

Human interleukin-11 (hIL-11) is a pleiotropic cytokine that is involved in numerous biological activities such as hematopoiesis, osteoclastogenesis, neurogenesis and female fertility. IL-11 is obviously a key reagent to study the IL-11 receptors. However, conventional radio-iodination techni-ques lead to a loss of IL-11 bioactivity. Here, we report the construction and the production of a new recombinant human IL-11 (FPDIL-11). In this molecule, a speci®c phosphorylation site (RRASVA) has been introduced at the N-terminus of rhIL-11. ...

8p research12 29-04-2013 32 4   Download

When studiedin vitro, mitochondrial permeability transition (MPT) is associated with an increase in mitochondrial per-meability to solutes up to 1500 Da in mass and a loss of electrical potential difference across the inner mitochondrial membrane (Dwmit). The MPT has been implicated as being important in cellular calcium homeostasis, autophagy and cell death via necrosis and apoptosis.

8p research12 23-04-2013 48 3   Download

Reactionof certainpeptides andproteinswithsingletoxygen (generated by visible light in the presence of rose bengal dye) yields long-lived peptide and protein peroxides. Incubation of these peroxides with glyceraldehyde-3-phosphate dehy-drogenase, in the absence of addedmetal ions, results in loss of enzymatic activity. Comparative studies with a range of peroxides have shown that this inhibition is concentration, peroxide, and time dependent, with H2O2less ecient than some peptide peroxides.

10p research12 23-04-2013 39 4   Download

Insect pests and pathogens (fungi, bacteria and viruses) are responsible for severe crop losses. Insects feed directly on the plant tissues, while the pathogens lead to damage or death of the plant. Plants have evolved a certain degree of resistance through the production of defence compounds, which may be aproteic, e.g. antibiotics, alkaloids, terpenes, cyanogenic glucosides or proteic, e.g. chitinases,b-1,3-glu-canases, lectins, arcelins, vicilins, systemins and enzyme inhibitors.

16p research12 23-04-2013 44 3   Download

Using 0.4Mimidazole citrate buffer (pH 7.5) containing 0.1 mML-cysteine, homodimeric starch phosphorylase from Corynebacterium calluane(CcStP) was dissociated into native-like folded subunits concomitant with release of pyridoxal 5¢-phosphate and loss of activity. The inactivation rate of CcStP under resolution conditions at 30
Cwas, respectively, four- and threefold reduced in two mutants, Arg234fiAla and Arg242fiAla, previously shown to cause thermostabilization of CcStP [Griessler, R., Schwarz, A., Mucha, J.&Nidetzky, B. ...

11p awards 05-04-2013 44 5   Download

In this study we investigate the active-site structure and the catalytic mechanism of clostripain by using a combination of three separate techniques: affinity labelling, site-directed mutagenesis and molecular modelling. A benzamidinyl-diazo dichlorotriazine dye (BDD) was shown to act as an efficient active site-directed affinity label for Clostridium histolyticumclostripain. The enzyme, upon incubation with BDD in 0.1MHepes/NaOHbuffer pH 7.6, exhibits a time-dependent loss of activity.

10p dell39 03-04-2013 55 3   Download

The inner nuclear membrane protein MAN1 has been identified as an important factor in transforming growth factor b⁄bone morphogenic pro-tein (TGFb⁄BMP) signaling. Loss of MAN1 results in three autosomal dominant diseases in humans; all three characterized by increased bone density.

9p galaxyss3 21-03-2013 47 3   Download

Cadherins belong to a family of homophilic cell–cell adhesion proteins that are responsible for the establishment of a precise cell architecture and tissue integrity. Moreover, experimental data suggest that loss of intercellular adhesion is inversely correlated with cellular differentiation. Furthermore, dedifferentiation is closely linked to tumor progression.

13p galaxyss3 07-03-2013 36 2   Download

Proprotein convertase subtilisin⁄kexin 9 (PCSK9) is a secreted glycoprotein that regulates the degradation of the low-density lipoprotein receptor. Sin-gle nucleotide polymorphisms in its gene associate with both hypercholes-terolemia and hypocholesterolemia, and studies have shown a significant reduction in the risk of coronary heart disease for ‘loss-of-function’ PCSK9 carriers.

14p galaxyss3 07-03-2013 31 2   Download

The yeast 20S proteasome is subject to sulfhydryl redox alterations, such as the oxidation of cysteine residues (Cys-SH) into cysteine sulfenic acid (Cys-SOH), followed by S-glutathionylation (Cys-S-SG). Proteasome S-glutath-ionylation promotes partial loss of chymotrypsin-like activity and post-acidic cleavage without alteration of the trypsin-like proteasomal activity.

14p galaxyss3 07-03-2013 36 5   Download

Rare, inherited mutations causing familial forms of Parkinson’s disease have provided insight into the molecular mechanisms that underlie the genetic and sporadic forms of this disease. Loss of protein function result-ing from autosomal-recessive mutations in PTEN-induced putative kinase 1 (PINK1), Parkin and DJ-1 has been linked to mitochondrial dysfunction, accumulation of abnormal and misfolded proteins, impaired protein clear-ance and oxidative stress.

9p vinaphone15 28-02-2013 40 3   Download

Induction of heat shock proteins (HSPs) helps cells to survive severe hyper-thermal stress and removes toxic unfolded proteins. At the same time, the cap-dependent translation of global cellular mRNA is inhibited, due to the loss of function of eukaryotic initiation factor (eIF)4F complex.

19p vinaphone15 27-02-2013 22 2   Download

Metronidazole (MTZ) is widely used in combination therapies against the human gastric pathogenHelicobacter pylori. Resistance to this drug is com-mon among clinical isolates and results from loss-of-function mutations in rdxA, which encodes an oxygen-insensitive nitroreductase. The RdxA-associated MTZ-reductase activity ofH. pyloriis lost upon cell disruption. Here we provide a mechanistic explanation for this phenomenon.

11p viettel02 22-02-2013 50 3   Download

Recent reports have demonstrated that interactions between the microtu-bule-associated protein tau and the nonreceptor tyrosine kinase Fyn play a critical role in mediating synaptic toxicity and neuronal loss in response to b-amyloid (Ab) in models of Alzheimer’s disease.

11p cosis54 04-01-2013 43 5   Download

Alzheimer’s disease is the most common form of dementia and its patho-logical hallmarks include the loss of neurones through cell death, as well as the accumulation of amyloid fibres in the form of extracellular neuritic pla-ques.

13p cosis54 09-12-2012 41 3   Download

Lack of knowledge and understanding at the scientific, extension and farmer levels is the primary limitation to the effective management of Phytophthora diseases in Vietnam. The objectives of this project are to extend sustainable and effective disease control and management recommendations to a range of horticultural crops throughout Vietnam to reduce crop losses due to Phytophthora and to improve farmer incomes.

30p tam_xuan 02-03-2012 69 9   Download

"Investigation and control of the rice in the field and during harvest in the Mekong Delta of Vietnam" project is part of the Cooperation Programme for Agriculture and Rural Development (CARD) between the Ministry of Agriculture and rural Development of Vietnam (Ministry of Agriculture and rural Development) VACO Australian International Development (AusAID). The purpose of this project is to help households and small farmers in Vietnam to improve rice quality and prices to improve their income. The project will be implemented through a cooperative choice.

9p xinh_la 10-02-2012 97 7   Download

Abstract To overcome loss of stem-like properties and spontaneous differentiation those hinder the expansion and application of human mesenchymal stem cells (hMSCs), we have clonally isolated permanent and stable human MSC lines by ectopic overexpression of primary cell cultures of hMSCs with HPV 16 E6E7 and human telomerase reverse transcriptase (hTERT) genes. These cells were found to have a differentiation potential far beyond the ordinary hMSCs. They expressed trophoectoderm and germline specific markers upon differentiation with BMP4 and retinoic acid, respectively.

13p toshiba23 18-11-2011 54 3   Download