Molecular techniques

IHC has became an indispensable tool adjunct to the conventional pathology in daily practice. This technique is called immunohistochemistry because it was combined by two aspects including immunology and histochemistry applied directly on the tissue (taken from the patient lesion) section embedded in paraffin.

4p viuchiha 06-01-2025 1 1   Download

Leukemia is the most common childhood cancer in both Vietnam and another country around the world. Although the rate of successful treatment has increased due to the improvements of therapy and supportive care, the rate of relapse is still high.

5p viuchiha 06-01-2025 4 1   Download

Polymerase Chain Reaction is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified to be able to a identify, manipulate DNA, detect infectious organisms, including the viruses that cause AIDS, hepatitis, tuberculosis, detect genetic variations, including mutations, in human genes and numerous other tasks.

62p zingzing09 24-04-2013 58 7   Download

The incidence of hypervirulent epidemic Clostridium difficile has increased around the world and now in Australia. Assays that are capable of rapidly identifying these strains would enable earlier diagnosis and timely infection control response. The aims of the first part of the study were to validate and develop a molecular technique for the rapid diagnosis of toxigenic C.

134p runthenight07 01-03-2023 10 3   Download

In this Masters thesis computational modelling techniques were employed to investigate iron-free apo-Lactoferrin (apo-Lf) structural conformation changes in the presence of variant temperature and pH. These conditions represent the environment most milk protein goes through in food processing and the production of food products.

162p runthenight04 02-02-2023 9 3   Download

Campylobacter species are a leading cause of food-borne disease and C. jejuni highlight the most potential public health impact of Campylobacter contamination by pathogens originating from animals or animal products.. The total of 33meat samples comprising 8 from sheep (5) and goat (3) also 25 beef samples were screened by morphological, biochemical and molecular technique.

9p chauchaungayxua11 23-03-2021 23 3   Download

Research objectives: Thesis was the first studied methodically systematic assessment of genetic diversity by morphological indicators combined with molecular markers, identification of Vietnamese native Dendroium species based on the ITS sequences. The results of the thesis have significance in the classification, the conservation service, and selection and breed new varieties; these will be contributing to improve Vietnam orchids.

27p change03 06-05-2016 74 6   Download

Cytochrome c is widely distributed in bacterial species, from mesophiles to thermophiles, and is one of the best-characterized redox proteins in terms of biogenesis, folding, structure, function, and evolution. Experimental molecular biology techniques (gene cloning and expression) have become applicable to cytochrome c, enabling its engineering and manipulation. Heterologous expression systems for cytochromes c in bacteria, for use in mutagenesis studies, have been established by extensive investigation of the biological process by which the functional structure is formed. ...

7p research12 01-06-2013 36 3   Download

A comparative study of thermodynamic and kinetic aspects of Cu(II) and Ni(II) binding at the N-terminal binding site of human and bovine serum albumins (HSA and BSA, respectively) and short peptide analogues was performed using potentiometry and spectroscopic techniques. It was found that while qualitative aspects of interaction (spectra and structures of complexes, order of reactions) could be reproduced, the quantitative parameters (stability and rate constants) could not.

9p research12 01-06-2013 44 4   Download

A sialylated lacto-N-neotetraose (Sial-lNnT) structural unit was identified and structurally characterized in the lipo-polysaccharide (LPS) from the genome-sequenced strain Road (RM118) of the human pathogenHaemophilus influ-enzaegrown in the presence of sialic acid. A combination of molecular genetics, MS and NMR spectroscopy techniques showed that this structural unit extended from the proximal heptose residue of the inner core regionof theLPSmolecule.

11p research12 23-04-2013 36 2   Download

This study describes the construction of a library of single-chain antibody fragments (scFvs) from a single human donor by individual amplification of all heavy and light variable domains (1.1·10 8 recombinants). The lib-rary was panned using the phage display technique, which allowed selection of specific scFvs (3F and C1) capable of recognizing Cn2, the major toxic component of Centruroides noxiusscorpion venom.

11p awards 06-04-2013 52 4   Download

The novel sex-specific potassium channel inhibitor IsTX, a 41-residue peptide, was isolated from the venom of male Opisthacanthus madagascariensis.Two-dimensionalNMR techniques revealed that the structure of IsTX contains a cysteine-stabilizeda/b-fold. IsTX is classified, based on its sequential and structural similarity, in the scorpion short toxin familya-KTx6. Thea-KTx6 family contains a single a-helix and twob-strands connected by four disulfide brid-ges and binds to voltage-gated K + channels and apamin-sensitive Ca 2+ -activated K + channels. ...

0p awards 05-04-2013 36 3   Download

In this study we investigate the active-site structure and the catalytic mechanism of clostripain by using a combination of three separate techniques: affinity labelling, site-directed mutagenesis and molecular modelling. A benzamidinyl-diazo dichlorotriazine dye (BDD) was shown to act as an efficient active site-directed affinity label for Clostridium histolyticumclostripain. The enzyme, upon incubation with BDD in 0.1MHepes/NaOHbuffer pH 7.6, exhibits a time-dependent loss of activity.

10p dell39 03-04-2013 55 3   Download

We have used footprinting techniques on a wide range of natural and synthetic footprinting substrates to examine the sequence-selective interaction of the bis-daunorubicin anti-biotic WP631 with DNA. The ligand produces clear DNase I footprints that are very different from those seen with other anthracycline antibiotics such as daunorubicin and nogalamycin. Footprints are found in a diverse range of sequences, many of which are rich inGT (AC) or GA (TC) residues.

11p dell39 03-04-2013 64 3   Download

Bermuda grass pollen (BGP) contains a very complex mixture of allergens, but only a few have been characterized. One of the allergens, with an apparent molecular mass of 21 kDa, has been shown to bind serum IgE from 29% of patients with BGP allergy. A combination of chromato-graphic techniques (ion exchange and reverse phase HPLC) was used to purify the 21 kDa allergen.

10p dell39 27-03-2013 60 3   Download

Many branchiopod crustaceans are endowed with extracellular, high-molecular-weight hemoglobins whose exact structural characteristics have remained a matter of conjecture. By using a broad spectrum of techniques, we provide precise and coherent information on the hemoglobin of one of the phylogenetically ‘oldest’ extant branchiopods, the tadpole shrimp Triops cancriformis.

17p inspiron33 25-03-2013 45 5   Download

Heat shock inducible lysyl-tRNA synthetase ofEscherichia coli(LysU) is known to be a highly efficient diadenosine 5¢,5¢¢¢-P 1 ,P 4 -tetraphosphate (Ap4A) synthase. However, we use an ion-exchange HPLC technique to demonstrate that active LysU mixtures actually have a dual catalytic activ-ity, initially producing Ap4 A from ATP, before converting that tetraphos-phate to a triphosphate.

11p inspiron33 25-03-2013 39 3   Download

The crustacean hyperglycemic hormone (CHH) plays an important role in the regulation of hemolymph glucose levels, but it is also involved in other functions such as growth, molting and reproduction. In the present study we describe the first CHH family gene isolated from the Atlantic Ocean shrimp Litopenaeus schmitti.

9p inspiron33 23-03-2013 43 4   Download

A chilly, but still very attractive, Asilomar hosted the seventh edition of the Critical Assessment of Protein Structure Prediction Experiment (CASP) [1]. A few months ago, 96 protein sequences of unknown, but soon to be determined structure, were distributed to more than 250 groups, whose task was to predict their structure and a number of other fea-tures, such as their domain boundaries, the presence of disordered regions, contacts between specific amino acids and their molecular function.

4p galaxyss3 21-03-2013 48 4   Download

One of the important peptide hormones that control reproduction in crus-taceans is gonad-inhibiting hormone (GIH). GIH is known to modulate gonad maturation by inhibiting synthesis of vitellogenin (Vg), the precursor of yolk proteins. In this study, a cDNA encoding a GIH (Pem-GIH) from the eyestalk of Penaeus monodonwas cloned using RT-PCR and RACE techniques.

11p media19 06-03-2013 24 4   Download