Monomeric

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82p zhangyan 13-07-2021 20 6   Download

The most effective concentration of MSA was 5 mg L-1 at the pH range of 3.5 to 5 and electrolyte background of 0.01 molc L-1. Out of this pH range or at higher electrolyte backgrounds, clay suspension is more strongly favored or prohibited; the effect of MSA was usually hidden.

7p nguyenvanhoangvnu 12-06-2017 55 2   Download

The case of homologous monomeric c-type and oligomeric b-type crystallins has been described and analyzed in evolutionary terms. Data and hypotheses from molecular genetics and structural investigations converge and suggest a novel three-phase model for the evolutionary history of crystallin-type proteins. In the divergent cascades of monomeric and oligomeric crystallins, a pivotal role was played by alterations in the gene segments encoding the C-terminal extensions and the intermotif or interdomain linker peptides.

9p system191 01-06-2013 41 4   Download

Recombinant human kynureninase (L-kynurenine hydrolase, EC 3.7.1.3) was purified to homogeneity (60-fold) from Spodoptera frugiperda (Sf9) cells infected with baculovirus containing the kynureninase gene. The purification protocol comprised ammonium sulfate precipitation and several chromatographic steps, including DEAE–Sepharose CL-6B, hydroxyapatite, strong anionic and cationic separations. The purity of the enzyme was determined by SDS/ PAGE, and the molecular mass verified by MALDI-TOF MS. The monomeric molecular mass of 52.4 kDa determined was 99.99% of the predicted molecular mass.

6p research12 01-06-2013 49 5   Download

Immunoblotting analyses revealed the presence of carb-oxylesterase in theporcine small intestine, liver, submaxillary andparotidglands, kidney cortex, lungs and cerebral cortex. In the intestinal mucosa, the pI 5.1 enzyme was detected in several subcellular fractions including the microvillar frac-tion. Both fatty monoacylated and diacylated monomeric (F1), trimeric (F3) and tetrameric (F4) forms of the intestinal protein were puri®ed here for the ®rst time by performing hydrophobic chromatography and gel ®ltration....

9p research12 01-06-2013 41 6   Download

Primary structural information including the complete nucleotide sequence of the ®rst insect aldehyde oxidase (AO) was obtained from the common house mosquitoCulex quinquefasciatus(Say) through cloning and sequencing of both genomic DNA and cDNA. The deduced amino-acid sequence encodes a 150-kDa protein of 1266 amino-acid residues, which is consistent with the expected monomeric subunit size of AO.

12p research12 01-06-2013 48 5   Download

Vibrio choleraehemolysin (HlyA), a water-soluble protein with a native monomeric relative molecular mass of 65 000, forms transmembrane pentameric channels in target bio-membranes.TheHlyAbinds to lipid vesicles nonspecifically and without saturation; however, self-assembly is triggered specifically by cholesterol.Here we show that the HlyA partitioned quantitatively to amphiphilic media irrespective of their compositions, indicating that the toxin had an amphiphilic surface.

8p research12 23-04-2013 56 4   Download

Human butyrylcholinesterase (BChE; EC 3.1.1.8) is of particular interest because it hydrolyzes or scavenges a wide range of toxic compounds including cocaine, organophos-phorus pesticides andnerveagents.The relative contribution of each N-linked glycan for the solubility, the stability and the secretionof the enzymewas investigated. Arecombinant monomeric BChE lacking four out of nine N-glycosylation sites and the C-terminal oligomerization domain was stably expressed as a monomer in CHO cells.

8p research12 23-04-2013 29 4   Download

In the leukemic macrophage cell-line THP-1, a fraction of the secreted matrix metalloproteinase 9 (MMP-9) is linked to the core protein of chondroitin sulfate proteoglycans (CSPG). Unlike the monomeric and homodimeric forms of MMP-9, the addition of exogenous CaCl2 to the proMMP-9/CSPG complex resulted in an active gelatinase due to the induction of an autocatalytic removal of the N-terminal prodomain.

12p tumor12 20-04-2013 35 3   Download

Duckd2-crystallin is a soluble tetrameric lens protein. In the presence of guanidinium hydrochloride (GdnHCl),it undergoes stepwise dissociation and unfolding. Gel-filtra-tion chromatography and sedimentation velocity analysis has demonstrated the dissociation of the tetramer protein to a monomeric intermediate with a dissociation constant of 0.34lM 3 . Dimers were also detected during the dissociation and refoldingprocesses.

8p tumor12 20-04-2013 33 1   Download

The structure of CLIC4, a member of the CLIC family of putative intracel-lular chloride ion channel proteins, has been determined at 1.8 A ˚ resolution by X-ray crystallography. The protein is monomeric and it is structurally similar to CLIC1, belonging to the GST fold class. Differences between the structures of CLIC1 and CLIC4 are localized to helix 2 in the glutaredoxin-like N-terminal domain, which has previously been shown to undergo a dra-matic structural change in CLIC1 upon oxidation.

12p fptmusic 12-04-2013 35 3   Download

Bifidobacteriumbifidumis a useful probiotic agent exhibiting health-promoting properties and containsD-aspartate as an essential component of the cross-linker moiety in the pepti-doglycan. To help understandD-aspartate biosynthesis in B. bifidumNBRC 14252, aspartate racemase, which cata-lyzes the racemization ofD-andL-aspartate, was purified to homogeneity and characterized. The enzyme was a mono-mer with amolecularmass of 27 kDa. This is the first report showing the presence of a monomeric aspartate racemase....

0p awards 05-04-2013 42 2   Download

We reported previously that the major cysteine protease in embryos and larvaeof thebrine shrimp,Artemia franciscana, is a heterodimeric protein consisting of a catalytic subunit (28.5 kDa) with a high degree of homology with cathep-sin L, and a noncatalytic subunit (31.5 kDa) of unknown function. In the study reportedhere thenoncatalytic subunit, or cathepsin L-associated protein (CLAP), was separated from cathepsin L by chromatography on Mono S and found tocontainmultiple isoformswithpIs ranging from5.9 to 6.1.

12p awards 05-04-2013 54 4   Download

Iron–sulfur clusters are widely represented in most organ-isms, but the mechanism of their formation is not fully understood. Of the two main proteins involved in cluster formation, NifS/IscS and NifU/IscU, only the former has been well studied from a structural point of view. Here we report anextensive structural characterizationofEscherichia coliIscU. We show by a variety of physico-chemical tech-niques thatE. coliIscU construct can be expressed to high purity as a monomeric protein, characterized by anabfold withhigha-helix content. ...

8p dell39 03-04-2013 42 5   Download

We studied the interaction of several metal ions with the copper chaperone fromEnterococcus hirae(EhCopZ). We show that the stoichiometry of the protein–metal complex varies with the experimental conditions used. At high con-centration of the protein in a noncoordinatingbuffer, a dimer, (EhCopZ)2–metal, was formed. The presence of a potentially coordinatingmolecule L in the solution leads to the formation of a monomeric ternary complex, EhCopZ– Cu–L, where L can be a buffer or a coordinatingmole-cule (glutathione, tris(2-carboxyethyl)phosphine). ...

11p dell39 03-04-2013 52 6   Download

We have investigated the molecular mechanisms that produce different structural and functional behavior in the monomeric and trimeric forms of seminal vesicle protein no. 4, a protein with immunomodulatory, anti-inflamma-tory, and procoagulant activity secreted from the rat seminal vesicle epithelium. The monomeric and trimeric forms were characterized in solution by CD.

9p dell39 03-04-2013 34 5   Download

Purified human Rad51 and Rad52 proteins exhibit multiple oligomeric states, in vitro. Single-stranded DNA (ssDNA) renders high molecular weight aggregates of both proteins into smaller and soluble forms that include even the monomers. Consequently, these proteins that have a pro-pensity to interact with each other’s higher order forms by themselves, start interacting with monomeric forms in the presence of ssDNA, presumably reflecting the steps of protein assembly on DNA.

9p dell39 27-03-2013 32 4   Download

The amyloidbpeptide (Ab) is crucial for the pathogenesis of Alzheimer’s disease. Aggregation of monomeric Ab into insoluble amyloid fibrils proceeds through several soluble Abintermediates, including protofibrils, which are believed to be central in the disease process. The main reason for this is their implication in familial Alzheimer’s disease with the Arctic amyloid precursor protein mutation (E693G).

13p inspiron33 26-03-2013 44 3   Download

The gene encoding RNase HII from the psychrotrophic bacterium,Shewa-nellasp.SIB1was cloned, overexpressed inEscherichia coli, and the recom-binant protein was purified and biochemically characterized. SIB1 RNase HII is a monomeric protein with 212 amino acid residues and shows an amino acid sequence identity of 64% to E. coliRNase HII.

12p inspiron33 26-03-2013 41 4   Download

Parkia platycephalalectin 2 was purified from Parkia platycephala(Legumi-nosae, Mimosoideae) seeds by affinity chromatography and RP-HPLC. Equilibrium sedimentation and MS showed that Parkia platycephala lectin 2 is a nonglycosylated monomeric protein of molecular mass 29 407 ± 15 Da, which contains six cysteine residues engaged in the for-mation of three intramolecular disulfide bonds.

13p inspiron33 25-03-2013 31 3   Download