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Báo cáo khoa học: Purification and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:6

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Salt-active acharan sulfate lyase (no EC number) has been purified fromBacteroides stercorisHJ-15,which was iso-lated from human intestinal bacteria with GAG degrading enzymes. The enzymewas purified toapparent homogeneity by a combination of QAE-cellulose,diethylaminoethyl (DEAE)-cellulose,CM-Sephadex C-50, HA ultrogel and phosphocellulose column chromatography with the final specific activity of 81.33lmolÆmin )1 Æmg )1 .

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Nội dung Text: Báo cáo khoa học: Purification and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15

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