Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays
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An activity that inhibited both glutamine synthetase (GS) and nitrate reductase (NR) was highly purified from cauli-flower (Brassica oleraceavar.botrytis) extracts.The final preparationcontainedanacyl-CoAoxidase andasecond proteinof theplant nucleotidepyrophosphatase family.This preparationhydrolysedNADH, ATPandFAD togenerate AMP andwas inhibited by fluoride, Cu 2+ ,Zn 2+ andNi 2+ .
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