BÁO CÁO: SỬ DỤNG KỸ THUẬT DAS-ELISA VÀ RT-PCR ĐỂ PHÁT HIỆN VIRUS GÂY BỆNH ĐỐM VÕNG TRÊN CÂY ĐU ĐỦ (Papaya ringspot virus) TẠI HAI TỈNH ĐỒNG NAI VÀ ĐỒNG THÁP (part 10)

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Nhƣ đã nêu ở phần Vật liệu và Phƣơng pháp, các mẫu sử dụng trong thí nghiệm RT-PCR là các mẫu dƣơng tính chọn lọc từ kết quả của test kit ELISA. Với kết quả dƣơng tính thu đƣợc từ phƣơng pháp RT-PCR đã củng cố và bổ sung cho kết quả thu đƣợc từ phƣơng pháp ELISA. Nhận thấy, dù chẩn đoán bệnh bằng phƣơng pháp nào đi nữa thì độ tin cậy đều nhƣ nhau

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Nội dung Text: BÁO CÁO: SỬ DỤNG KỸ THUẬT DAS-ELISA VÀ RT-PCR ĐỂ PHÁT HIỆN VIRUS GÂY BỆNH ĐỐM VÕNG TRÊN CÂY ĐU ĐỦ (Papaya ringspot virus) TẠI HAI TỈNH ĐỒNG NAI VÀ ĐỒNG THÁP (part 10)

79 Phụ lục 12 Kết quả so với trình tự của CP-PRSV trên Genebank CLUSTAL X (1.83) multiple sequence alignment 3 ------------------------------------------------------------ AJ012649 TCCAAGAATGAAGCTGTGGATGCTGGTTTGGATGAAAAACTCAAAGAAAAAGAAAAACAG 60 3 ------------------------------------------------------------ AJ012649 AAAGAAAAAGAAAAACAAAAAGAAAAAGAAAAAGACAATGCTAGTGACGGAAATGATGTG 120 3 ------------------------------------------------------------ AJ012649 TCGACTAGCACAAGAACTGGAGAGAAAGATAGAGATGTCAATGTCGGGACCAGTGGAACT 180 3 1 ---------CCGAGAATAAAATCATTTACTGACAAGATGATTTTACCGAAAATTAAAGGA 51 AJ012649 181 TTCACGGTTCCGAGAATTAAATCATTCACTGATAAGATGATTCTACCAAGAATTAAGGGA 240 190******** ******** ***** ********* **** * ****** *** 3 AAAACTGTCCTTAATTTTAAATCATCTTCTTCAGTATAATCCGCAACAAATTGATATCTC 111 AJ012649 AAGACTGTCCTTAATTT-AAATCATCTTCTTCAGTATAATCCGCAACAAATTGACATTTC 299 ** ************** ************************************ ** ** 3 AAACACTCGTGCCACTCAAACTCAATTCGAAAAGTGGTATGAGGGAGTGAGGAATGATTA 171 AJ012649 TAACACTCGTGCCACTCAGTCACAATTTGAGAAATGGTATGAGGGAGTGAGGAATGATTA 359 ***************** * ***** ** ** ************************** 3 CGGTCTCAACGATAACGAAATGCAAGTG-ATGTTAAACGGTTTGATGGTTTGGTGTATTG 230 AJ012649 TGGTCTGAATGATAATGAAATGCAAGTG-ATGCTGAATGGCTTGATGGTTTGGTGTATCG 418 ***** ** ***** ************ *** * ** ** ***************** * 3 AAAATGGTACATCTCCAGACTTATCTGGTGTTTGGGTAATGATGGATGGGGAAACACAAG 290 AJ012649 AGAATGGTACATCTCCAGACATATCTGGTGTTTGGGTTATGATGGATGGGGAAATTCAAG 478 * ****************** **************** **************** **** 3 TTGATTATCCCATTAAGCCTTTAATTGAACATGCAACTCCTTCGTTTAGGCAAATCATGG 350 AJ012649 TTGACTATCCAATCAAGCCTTTAATTGAGCATGCTACCCCGTCATTTAGGCAGATTATGG 538 **** ***** ** ************** ***** ** ** ** ******** ** **** 3 351 CTCACTTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGGAATG--------------- 396 AJ012649 CTCACTTTAGTAACGCGGCAGAAGCATATATTGCAAAGAGAAATGCCACTGAGAGGTACA 598 ******* ************** ***** ** ******** **** 583 3 ------------------------------------------------------------ AJ012649 TGCCGCGGTATGGAATCAAGAGAAATTTGACTGACATTAGCCTCGCTAGATACGCTTTCG 3 ------------------------------------------------------------ AJ012649 ATTTCTATGAGGTTAATTCGAAAACACCTGATAGGGCTCGCGAAGCTCACATGCAGATGA 3 ------------------------------------------------------------ AJ012649 AAGCTGCAGCGCTGCGAAACACTAGTCGCAGAATGTTTGGTATGGACGGCAGTGTTAGTA 3 ------------------------------------------------------------ AJ012649 ATAAGGAAGAAAACACGGAGAGACACACAGTGGAAGATGTCAATAGAGACATGCACTCTC 3 ------------------------------------------------------------ AJ012649 TCCTGGGTATGCGCAACTGAATACTCGCGCTGGTGTGTTTGTCGAGCTCGACTCGACCTT 3 ------------------------------------------------------------ AJ012649 GTTTCACCTTATAGTACTAAATAAGCATTAGAATACAGTGTGGCTGTGCCACCACTTCTA 3 ------------------------------------------------------------ AJ012649 TTTTACAGTGAGGGTAGCCCTCCGTGCTTTTAGTATTATTCGAGTTCTCTGAGTCTCCAT 3 --------------------------- AJ012649 ACAGTGTGGGTGGCCCACGTGTTATTT ALIGN 396 NU
80 Phụ lục 13 Kết quả so với genome của PRSV trên Genebank CLUSTAL X (1.83) multiple sequence alignment 3 ------------------------------------------------------------ AY231130 1 AAATAAAACATCTCAACACAACATAATCAAAAGCATTTCAACAAACATCAACTTATCTCA 60 3 ------------------------------------------------------------ AY231130 TTTTAAATTGTTATAGTAAGCGACAATGTCTTCTTTGTATACTTTGCGAGCAGCAGCTCA 120 3 ------------------------------------------------------------ AY231130 ATACGATAGGAGGTTGGAGAGTAAGAAAGGTGCTGGCTGGATCGAGCACAAACTCGAGAG 180 3 ------------------------------------------------------------ AY231130 GAAAGGGGATAAAGGAAACACTCACTATTGTAGTGAGTTTGACATTAGCAAGGGTGCCAA 240 3 ------------------------------------------------------------ AY231130 AATTCTGCAATTGGTGCAAATTGGTAGCGCTGAAGTTGGAAGGACCTTCCTGGAGGGTAA 300 3 ------------------------------------------------------------ AY231130 CAGATTTGTCCGTGCGAACATATTTGAGATCATCCGGAAAACCATGGTTGGTCGTCTAGG 360 3 ------------------------------------------------------------ AY231130 ATATGATTTCGAGAGTGAACTATGGGTCTGTCATAACTGCAACAGAACTTCTGAAAAATA 420 3 ------------------------------------------------------------ AY231130 CCTCAAGAAATGTGACTGCGGGGAAAAATATTATTACTCTGAAAGAAACCTGATGAAAAC 480 3 ------------------------------------------------------------ AY231130 AATGAACGACCTGACGTATCAATTTGACATGACACCATCAGAGATCAACTCCGTCGATTT 540 3 ------------------------------------------------------------ AY231130 TGATTACCTTGCTGATGCAGTGGATTATGCTGAACGGTCAGTCCAGAAATCTCAAGTTCC 600 3 ------------------------------------------------------------ AY231130 GGAACCTGTGGAACTTGTAGTGGTGGAACCAATTGCGGCTAGTGAGGAAGGTACTCTAAT 660 3 ------------------------------------------------------------ AY231130 GGTTTCTGAACCAGAAGTTGTACCTGCAACTACCAAAATTGAAGATGCATGGACAATACA 720 3 ------------------------------------------------------------ AY231130 GATTGGGGAAATTCCTGTCCCACTTGTCGTCATCAAAGAGACGCCAGTTATCAGTGGTGT 780 3 ------------------------------------------------------------ AY231130 GAACGGAACTTTGAACTCAACTGGTTTCTCACTTGAAGCTGAGATTACAAAACTGGTGGA 840 3 ------------------------------------------------------------ AY231130 GAAGGAAGTCTCTCAAGAAGAGGTTAAAGAAGCAGTGCATCTGGCACTCGAAGTTGGTAA 900 3 ------------------------------------------------------------ AY231130 CGAGATTGCTGAGAAGAAGCCTGAACTCAAGCTAATACCATACTGGAGTGCTAGCCTTGA 960 3 ------------------------------------------------------------ AY231130 GTTGCACAAGAGAATTCGTAAGCATAAGGAGCATGCTAAGATTGCAGCAATTCAAGTCCA 1020 3 ------------------------------------------------------------ AY231130 GAAGGAAAGAGAAAAGAACCAAAAGATATTCTCAATTCTAGAGTCGAAGCTCAACTTAAA 1080 3 ------------------------------------------------------------ AY231130 ATCCAGGAGGAGAAATCAAACTGTGGTCTGTGACAAAAGAGGCACACTTAAATGGAAAAC 1140 3 ------------------------------------------------------------ AY231130 CCGGCAAGGTTACAAGAAGAGTAGACTAATGCAACAAGCGAGTGACTCTGTTGTCACTCA 1200
81 3 ------------------------------------------------------------ AY231130 GATTTACTGTGATTTTGGTTGTGAACCTCAATATTCTGAACCTCACTCTTCTGGCATTAA 1260 3 ------------------------------------------------------------ AY231130 AATAGCGACATCTAAGAAGGTCCACAAACCGCGCAAGCAATCGAGGATTGTTGGTAATAA 1320 3 ------------------------------------------------------------ AY231130 CAAGATAAATTATGTCATGAAACATCTATGTGACACAATTATCGAAAGAAGCATTCCTGT 1380 3 ------------------------------------------------------------ AY231130 AGAGCTTGTTACAAAGCGATGCAAGAGAAGGATTCTTCAGAAGAAAGGTAGAAGCTATGT 1440 3 ------------------------------------------------------------ AY231130 GCAGTTGAGGCACATGGATGGCATTCGTGCACAACAAGATGTGAGCAGCTCGCCTGATAT 1500 3 ------------------------------------------------------------ AY231130 GGAGCGGTTATTCACGCAATTTTGCAAATTTTTAGTTGGGCACAAACCACTCAAATCTGA 1560 3 ------------------------------------------------------------ AY231130 AAGTCTGACGTTTGGTTCTAGTGGTCTGATTTTTAAGCCAAAGTTTGCCGCCAATGTGGG 1620 3 ------------------------------------------------------------ AY231130 GCGATATTTTGGTGATTATTTCGTTGTTCGAGGACGTCTTGAAGGTAAGCTATTTGATGG 1680 3 ------------------------------------------------------------ AY231130 TAGATCAAAACTAGCGAGATCGATCTATGCCAGGATGGAGCAATACAATGATGTGGCCGA 1740 3 ------------------------------------------------------------ AY231130 AAAATTTTGGCTCGGTTTTAATAGGGCTTTTCTACGGCATAGAAAACCAACGGATCACAC 1800 3 ------------------------------------------------------------ AY231130 TTGCACGTCTGATATGGATGTTACAATGTGTGGGGAGGTAGCAGCTCTGGCAACTATAAT 1860 3 ------------------------------------------------------------ AY231130 TTTGTTTCCGTGCCACAAAATAACTTGCAACACTTGCATGAGCAGAGTGAAGGGAAGAGT 1920 3 ------------------------------------------------------------ AY231130 AATTGACGAAGTTGGTGAGGATTTGAATTCTGAGCTTGAACGTTTACGTGAAACTCTTTC 1980 3 ------------------------------------------------------------ AY231130 TTCATATGGAGGGTCATTCGGACATGTTTCAACATTACTTGATCAAATGAATAGAGTTTT 2040 3 ------------------------------------------------------------ AY231130 GAATGCGCGGAACACAAACGATGGAGCTTTTAAAGAGATTGCGAAGAAGATTGATGCAAA 2100 3 ------------------------------------------------------------ AY231130 GAAAGAGAGTCCTTGGACTCACATGACAACTATCAACAACACGCTCATCAAAGGTTCGCT 2160 3 ------------------------------------------------------------ AY231130 AGCAACTGGTTACGAATTTGAAAGAGCGTCTGATAGTCTCCTAGAGATTGTGAGATGGCA 2220 3 ------------------------------------------------------------ AY231130 TCTCAAAAGGACAGAATCAATAAAAGCTGGCAGCGTTGAGAGTTTTAGAAATAAGCGTTC 2280 3 ------------------------------------------------------------ AY231130 TGGGAAAGCTCACTTCAACCCAGCTCTTACGTGTGATAATCAGTTGGATAGGAATGGTAA 2340 3 ------------------------------------------------------------ AY231130 TTTCTTATGGGGTGAAAGGCAATACCACGCCAAAAGATTCTTTGCCAACTATTTCGAGAA 2400 3 ------------------------------------------------------------ AY231130 GATTGATCATAGTAAGGGTTATGAGTACTATAGTCAACGCCAAAACCCAAATGGTACCAG 2460 3 ------------------------------------------------------------ AY231130 GAAAATTGCCATTGGTAATTTAGTATTTTCAACAAATTTGGATAGATTTCGGCAGCAAAT 2520 3 ------------------------------------------------------------ AY231130 GGTTGAACACCACATTGATCAAGGACCAATCACTCGTGAGTGCATCGCATTGCGCAATAA 2580
82 3 ------------------------------------------------------------ AY231130 CAATTATGTTCATGTATGTAGCTGTGTAACTTTAGATGATGGAACTCCAGCAACAAGTGA 2640 3 ------------------------------------------------------------ AY231130 GTTGAAGACTCCTACCAAGAATCATATCGTTCTTGGTAATTCTGGTGATCCCAAGTACGT 2700 3 ------------------------------------------------------------ AY231130 TGACTTGCCGACTCTTGAGTCTGATTCAATGTACATAGCCAAAAAAGGTTATTGCTACAT 2760 3 ------------------------------------------------------------ AY231130 GAACATATTTTTAGCGATGCTCATAAATATACCTGAGAATGAGGCGAAAGATTTCACAAA 2820 3 ------------------------------------------------------------ AY231130 GAGAGTTCGCGACCTCGTGGGTTCAAAACTTGGAGAGTGGCCAACGATGTTGGATGTCGC 2880 3 ------------------------------------------------------------ AY231130 AACATGCGCAAATCAGCTGATAATCTTTCATCCTGACGCAGCCAATGCAGAATTGCCGCG 2940 3 ------------------------------------------------------------ AY231130 AATTTTAGTGGACCATCGACAGAAAACAATGCATGTTATTGACTCTTTTGGTTCCGTGGA 3000 3 ------------------------------------------------------------ AY231130 TTCTGGATATCATATGTTGAAGGCAAACACAGTTAATCAGCTAATCCAATTCGCTAGAGA 3060 3 ------------------------------------------------------------ AY231130 GCCACTCGATAGTGAAATGAAGCACTACATCGTTGGTGGAGAGTTTGACCCAACTACTAA 3120 3 ------------------------------------------------------------ AY231130 CTGCTTGCATCAGTTGATTCGTGTCATCTACAAGCCTCATGAACTCCGGAACTTGCTCAG 3180 3 ------------------------------------------------------------ AY231130 GAATGAACCGTACCTGATTGTGATTGCATTGATGTCACCAAGTGTACTTTTGACTTTGTT 3240 3 ------------------------------------------------------------ AY231130 CAATAGTGGTGCGATTGAGCACGCGTTGAATTACTGGATCAAAAGAGATCAAGATGTTGT 3300 3 ------------------------------------------------------------ AY231130 TGAGGTCATTGTTTTGGTGGAGCAATTATGCAGGAAAGTTACACTTGCTAGAACAATCCT 3360 3 ------------------------------------------------------------ AY231130 GGAGCAATTTAATGAGATTCGTCAAAATGCGAGAGATATACATGAGCTAATGGATCGAAA 3420 3 ------------------------------------------------------------ AY231130 CAATAAGCCTTGGATTTCATATGATCGCTCATTGGAACTATTGAGTGTGTATGCGAATTC 3480 3 ------------------------------------------------------------ AY231130 GCAGTTGACGGATGAAGGTCTACTCAAGCAAGGATTTTCAACATTAGATCCTAGGTTGCG 3540 3 ------------------------------------------------------------ AY231130 TGAAGCTGTGGAAAAAACCTACGCCACTCTTTTACAGGAAGAATGGCGTGCGTTAAGTTT 3600 3 ------------------------------------------------------------ AY231130 GTTTCAAAAGTTGCACTTAAGGTACTTTGCGTTCAAATCACAACCGTCTTTTTCCGAGTA 3660 3 ------------------------------------------------------------ AY231130 TTTAAAGCCAAAAGGGCGCGCAGATTTAAAAATTGTATACGACTTCTCACCGAAGTATTG 3720 3 ------------------------------------------------------------ AY231130 TGTACACGAGGTCGGAAAGGCGTTTCTACAACCAGTCAGGGCTGGGGCTAAAATTGCATC 3780 3 ------------------------------------------------------------ AY231130 GCGAGTCATTAATGGTTGCGGAACTTTCATTCGGAAGAGTGCCGCAAAAGGCTGTGCTTA 3840 3 ------------------------------------------------------------ AY231130 CATTTTTAAGGATCTTTTCCAGTTTGTACATGTAGTGTTAGTTTTAAGCATTTTATTACA 3900 3 ------------------------------------------------------------ AY231130 GATCTTTAGGAATGCGCAAGGAATTGCCACAGAGCATATACAATTGAAGCAGGCTAAGGC 3960
83 3 ------------------------------------------------------------ AY231130 AGAAATGGAAAGACAGAAAGATTTTGATCGTCTGGAGGCTCTATACGCTGAATTGTGCAT 4020 3 ------------------------------------------------------------ AY231130 TAAGAGCGGTGAGCAACCAACTGCTGAAGAATTTATTGATTTTGTGAAGGAGCGTGAGCC 4080 3 ------------------------------------------------------------ AY231130 AAGGCTTAAGGATCAAGCTTACAATTTAATACACATACCAGTGATTCATCAAGCAAAATC 4140 3 ------------------------------------------------------------ AY231130 GGACAATGAGAAAAAGCTCGAGCAAGTCATTGCATTCATTACATTAATTTTAATGATGAT 4200 3 ------------------------------------------------------------ AY231130 CGATGTTGATAAGAGTGATTGTGTCTATAGAATCCTAAATAAATTTAAAGGCGTGATAAA 4260 3 ------------------------------------------------------------ AY231130 ATCTTGCGATGCAAATGTTTATCACCAGTCTTTAGATGACATTCGGGATTTCTATGAAGA 4320 3 ------------------------------------------------------------ AY231130 CAAGCAGTTGACTATTGATTTCGATATCACTGGAGAAAATCAGATCAATCGAGGACCTAT 4380 3 ------------------------------------------------------------ AY231130 AGACGTTACGTTTGAGAAATGGTGGGACAATCAATTGTCCAATAACAACACAATTGGTCA 4440 3 ------------------------------------------------------------ AY231130 TTATCGAATCGGGGGAACGTTTGTTGAATTTTCACGAAGTACTGCAGCAACTGTGGCTAG 4500 3 ------------------------------------------------------------ AY231130 TGAGATAGCTCATAGTCCTGATCGTGAGTTTCTTGTTCGCGGAGCTGTTGGTAGTGGTAA 4560 3 ------------------------------------------------------------ AY231130 ATCAACGAATCTTCCTTTCTTGCTTAGTAAGCATGGTAGCGTGTTGTTAATAGAGCCCAC 4620 3 ------------------------------------------------------------ AY231130 CAGACCTCTTTGTGAGAACGTCTGTAAGCAATTACGTGGTGAACCGTTCCATTGTAATCC 4680 3 ------------------------------------------------------------ AY231130 AACCATTCGCATGCGTGGATTAACAGCTTTTGGTTCTACCAACATCACAATAATGACAAG 4740 3 ------------------------------------------------------------ AY231130 CGGATTTGCTTTACATTATTATGCTCACAATGTTCAACAATTGAGACTCTTTGATTTCAT 4800 3 ------------------------------------------------------------ AY231130 AATCTTTGATGAATGTCATGTCATAGATAGCCAAGCCATGGCTTTCTATTGTCTGATGGA 4860 3 ------------------------------------------------------------ AY231130 GGGGAACGCTATTGAGAAAAAGATCCTTAAAGTTTCTGCTACGCCACCTGGGCGTGAGGT 4920 3 ------------------------------------------------------------ AY231130 TGAATTTTCAACACAATTCCCAACAAAGATTGTAACGGAACAGTCCATAAGCTTTAAGCA 4980 3 ------------------------------------------------------------ AY231130 GTTGGTTGATAACTTTGGTACTGGTGCGAATAGTGATGTGACCGTTTTTGCTGATAACAT 5040 3 ------------------------------------------------------------ AY231130 ATTAGTTTATGTTGCCAGTTACAATGAAGTAGATCAACTAAGTAAGCTCTTATCCGATAA 5100 3 ------------------------------------------------------------ AY231130 AGGCTACTTAGTCACTAAAATCGATGGAAGAACGATGAAAGTTGGAAAAACTGAAATTTC 5160 3 ------------------------------------------------------------ AY231130 CACTAGTGGCACAAAATCCAAGAAGCATTTCATAGTTGCCACGAATATCATTGAGAATGG 5220 3 ------------------------------------------------------------ AY231130 CGTCACACTTGACATAGAAGCCGTTATAGATTTTGGGATGAAAGTTGTACCTGAGATGGA 5280 3 ------------------------------------------------------------
84 AY231130 TTCAGATAATCGCATGATTCGGTACTCAAAGCAAGCGATCAGTTTTGGAGAGAGAATTCA 5340 3 ------------------------------------------------------------ AY231130 AAGACTTGGCCGAGTGGGAAGACATAAAGAGGGGATTGCACTTAGAATTGGGCACACAGA 5400 3 ------------------------------------------------------------ AY231130 GAAAGGTATCCAAGAAATTCCAGAGATGGCAGCTACTGAAGCAGCTTTTCTGAGCTTCAC 5460 3 ------------------------------------------------------------ AY231130 GTATGGCTTGCCTGTTATGACTCACAATGTAGGGTTAAGCTTGCTTAAAAATTGCACTGT 5520 3 ------------------------------------------------------------ AY231130 GAGACAAGCACGCACAATGCATCAGTATGAACTGAGCCCATTCTTTACACAAAATTTGGT 5580 3 ------------------------------------------------------------ AY231130 AAATTTTGATGGGACAGTGCACCCCAAGATTGATATTCTGTTACGTCCCTATAAGCTGAG 5640 3 ------------------------------------------------------------ AY231130 AGATTGTGAAGTCAGATTAAGTGAAGCAGCAATTCCGCATGGGGTACAGTCTATTTGGTT 5700 3 ------------------------------------------------------------ AY231130 GTCTGCGCGGGATTATGAAGCAGTTGGAGGTCGTCTTTGCTTGGAGAGCGATGTTCGAA 5760 3 ------------------------------------------------------------ AY231130 ACCATTCCTCATTAAGGATGTTCCTGAGCGGTTATACAAAGAATTGTGGGATATCGTGCA 5820 3 ------------------------------------------------------------ AY231130 GACCTACAAGCGTGACTTTACATTTGGGCGAATTAATTCTGTGTCTGCTGGGAAAATTGC 5880 3 ------------------------------------------------------------ AY231130 GTACACATTAAGGACTGATGTGTATTCTATTCCTAGAACTCTCATAACGATTGACAAGCT 5940 3 ------------------------------------------------------------ AY231130 GATTGAGAGTGAAAACATGAAGCATGCCCATTTTAAAGCCATGACAAGTTGCACTGGCTT 6000 3 ------------------------------------------------------------ AY231130 AAACTCCAGCTTTTCTCTCCTTGGTGTTATAAACACTATCCAGAGTAGATATCTAGTTGA 6060 3 ------------------------------------------------------------ AY231130 TCATTCAGTTGAAAATATCAGGAAACTTCAACTAGCGAAGGCCCAGATTCAACAACTTGA 6120 3 ------------------------------------------------------------ AY231130 AGCTCATGTGCAAGAAAACAATGTTGAAAATTTAATTCAATCTCTTGGTGCTGTTAGAGC 6180 3 ------------------------------------------------------------ AY231130 TGTTTACCATCAAAGTGTTGATGGAGTCAAGCACATAAAGCGAGAGTTGGGCTTGAAGGG 6240 3 ------------------------------------------------------------ AY231130 AATTTGGGATGGTTCATTGATGATCAAGGATGCGATTGTATGCGGTTTCACAATGGCTGG 6300 3 ------------------------------------------------------------ AY231130 CGGTGCGATGCTTTTGTACCAACACTTTCGTGATAGGCTTACAAATGTTCATGTGTTTCA 6360 3 ------------------------------------------------------------ AY231130 CCAAGGTTTCTCTGCGCGACAGAGACAAAAGTTAAGATTTAAGTCAGCAGCAAATGCTAA 6420 3 ------------------------------------------------------------ AY231130 GCTTGGTCGAGAGGTCTATGGAGATGATGGAACAATTGAGCACTACTTTGGAGAGGCGTA 6480 3 ------------------------------------------------------------ AY231130 CACGAAGAAAGGAAACAAGAAAGGAAAGATGCATGGCATGGGCGTTAAAACGAGGAAATT 6540 3 ------------------------------------------------------------ AY231130 TGTTGCGACATATGGATTTAAACCGGAGGATTACTCGTATGTGCGGTACTTGGATCCTTT 6600 3 ------------------------------------------------------------ AY231130 AACAGGTGAGACTTTAGATGAAAGCCCACAGACTGATATCTCAATGGTGCAAGAACATTT 6660 3 ------------------------------------------------------------
85 AY231130 TGGTGAAATTCGGAATAAGTACATGGATTCAGACAGCTTCGACAGGCAAGCTTTGATAGC 6720 3 ------------------------------------------------------------ AY231130 AAACAATACAATTAAGGCCTATTATGTCCGAAACTCCGCGAAGACAGCATTGGAAGTTGA 6780 3 ------------------------------------------------------------ AY231130 TCTGACACCGCACAACCCTCTTAAAGTTTGTGACAATAAGTTGACCATTGCAGGATTTCC 6840 3 ------------------------------------------------------------ AY231130 AGACAGGGAAGCTGAGCTGAGACAAACAGGCCCGCCCAGAACTATTCAAGTCAATCAAGT 6900 3 ------------------------------------------------------------ AY231130 GCCACCACCTTCAAGATCAGTTCATCATGAAGGAAAAAGTCTTTGTCAAGGCATGCGAAA 6960 3 ------------------------------------------------------------ AY231130 TTACAATGGCATAGCTTCTGTAGTTTGCCATTTGAAAAACACATCAGGAAAGGGGAAGAG 7020 3 ------------------------------------------------------------ AY231130 CTTATTTGGAATCGGATATAATTCATTCATCATTACCAACCGACATTTGTTCAAGGAGAA 7080 3 ------------------------------------------------------------ AY231130 TAATGGTGAACTTATAGTGAAATCCCAACACGGTAAGTTTGTCGTCAAGAACACCACGAC 7140 3 ------------------------------------------------------------ AY231130 GCTCCGAGTTGCTCCAGTTGGTAAGACTGATCTTTTAATTATTCAAATGCCGAAAGATTT 7200 3 ------------------------------------------------------------ AY231130 TCCTCCATTTCATAGCAGAGCTAGGTTTAGGGCCATGAAAGCTGGGGACAAGGTTTGCAT 7260 3 ------------------------------------------------------------ AY231130 GATAGGTGTTGATTACCAAGAGAATCATATCGCGAGCAAAGTGTCTGAAACTTCTATCAT 7320 3 ------------------------------------------------------------ AY231130 AGTGAGGGCACGGGAGATTTTGGATGCCACTGGATTTCCACGAATGACGGTGATTGCGG 7380 3 ------------------------------------------------------------ AY231130 CAATCCATTAGTTAGTGTTTCAGATGGTTTTATTGTTGGCTTGCATAGTCTGTCGACATC 7440 3 ------------------------------------------------------------ AY231130 AACTGGAGATCAAAATTTCTTCGCTAAAATACCCGCACAATTTGAAGAAAAGGTCCTGAA 7500 3 ------------------------------------------------------------ AY231130 GAAGATTGATGATTTAACTTGGAGCAAACACTGGAGCTATAATATTAATGAACTGAGTTG 7560 3 ------------------------------------------------------------ AY231130 GGGGGCTCTCAAAGTGTGGGAAAGTCGACCCGAAGCAATTTTTAACGCGCAAAAGGAAGT 7620 3 ------------------------------------------------------------ AY231130 TAATCAATTGAATGCCTTCGAGCAAAGTGGTAGTCGTTGGCTCTTTGACAAACTACACGG 7680 3 ------------------------------------------------------------ AY231130 CAATTTGAAAGGAGTAAGTTCCGCTCCTAGCAATTTGGTGACAAAGCACGTTGTTAAAGG 7740 3 ----------------------------------------------------------- AY231130 TATTTGTCCTCTCTTTAGAAACTATCTCGAGTGTGATAAAGAGGCGAAAGCTTTCTTTAG 7800 3 ------------------------------------------------------------ AY231130 TCCACTTATGGGTCACTACATGAAGAGTGTTCTGAGCAAGGAAGCATACATTAAGGATTT 7860 3 ------------------------------------------------------------ AY231130 ATTTAAATATTCAAGTGACATTGTCGTTGGAGAAGTCAACCATGATATTTTTGAGGATAG 7920 3 ------------------------------------------------------------ AY231130 TGTCGCGCAAGTTGTCGAACTGTTGAACGATCACGAGTGTTTTGAACTTGAATATATTAC 7980 3 ------------------------------------------------------------ AY231130 AGACAGTGAAGTGATTATTCAGGCCTTGAACATGGATGCAGCTGTTGGAGCCTTGTACAC 8040
86 3 ------------------------------------------------------------ AY231130 AGGAAAGAAAAGGAAATATTTTGAGGGTTCAACAGTGGAGCATAGACAAGCACTTGTGCG 8100 3 ------------------------------------------------------------ AY231130 GAAAAGCTGTGAGCGTCTCTATGAAGGGAGAATGGGAGTCTGGAATGGGTCGCTAAAGGC 8160 3 ------------------------------------------------------------ AY231130 TGAACTGAGACCAGCTGAAAAAGTGCTCGCGAAAAAGACAAGGTCATTTACAGCAGCTCC 8220 3 ------------------------------------------------------------ AY231130 TCTTGACACACTGTTAGGAGCCAAAGTCTGCGTTGATGATTTCAACAACTGGTTCTACAG 8280 3 ------------------------------------------------------------ AY231130 TAAGAACATGGAATGTCCATGGACCGTTGGGATGACAAAATTTTACAAAGGCTGGGATGA 8340 3 ------------------------------------------------------------ AY231130 GTTTCTGAAGAAGTTTCCTGAAGGCTGGGTGTATTGTGATGCAGATGGCTCCCAGTTCGA 8400 3 ------------------------------------------------------------ AY231130 TAGCTCATTGACACCATACTTGTTAAATGCTGTGTTGTCAATCCGGTTATGGGCAATGGA 8460 3 ------------------------------------------------------------ AY231130 GGATTGGGATATTGGAGAGCAAATGCTTAAGAATTTGTATGGGGAAATTACTTACACGCC 8520 3 ------------------------------------------------------------ AY231130 AATATTGACACCGGATGGGACAATCGTCAAGAAGTTCAAAGGAAATAATAGTGGTCAACC 8580 3 ------------------------------------------------------------ AY231130 TTCGACAGTTGTTGATAATACATTAATGGTTTTAATCACAATGTATTACGCACTACGGAA 8640 3 ------------------------------------------------------------ AY231130 GATTGGTTATGATACGAAGGCTCAAGAAGACATGTGTGTATTTTATATCAATGGTGATGA 8700 3 ------------------------------------------------------------ AY231130 CCTCTGTATTGCAATTCACCCGGATCATGAGCATGTTCTTGACTCATTCTCTAGTTCATT 8760 3 ------------------------------------------------------------ AY231130 TGCTGAGCTTGGGCTTAAGTATGACTTCACACAAAGGCACCGGAATAAACAGGATTTGTG 8820 3 ------------------------------------------------------------ AY231130 GTTTATGTCGCATCGAGGTATTCTGATCGATGACATTTACATTCCGAAACTTGAACCTGA 8880 3 ------------------------------------------------------------ AY231130 GCGAATTGTTGCAATTCTTGAATGGGACAAATCTAAGCTTCCAGAGCATCGATTAGAGGC 8940 3 ------------------------------------------------------------ AY231130 AATCACAGCAGCAATGATAGAGTCATGGGGTTATGGTGAGCTAACACACCAAATTCGTAG 9000 3 ------------------------------------------------------------ AY231130 ATTTTATCAATGGGTTCTTGAACAAGCTCCATTCAATGAGTTGGCGAAACAAGGAAGGGC 9060 3 ------------------------------------------------------------ AY231130 CCCATACGTCTCAGAAGTTGGATTAAGAAGATTGTATACGAGTGAACGTGGATCAATGGA 9120 3 ------------------------------------------------------------ AY231130 TGAATTGGAAGCGTATATAGATAAGTACTTTGAGCGTGAGAGGGGAGAATCACCCGAATT 9180 3 ------------------------------------------------------------ AY231130 ACTAGTGTATCATGAATCGAAGAGCACCGATGATTATCAACTTGTTTGTAATAACAATGC 9240 3 ------------------------------------------------------------ AY231130 ACATGTGTTTCATCAGTCAAAAAATGAAGCTGTGGATGCTGGTTTGAATGAAAAACTCAA 9300 3 ------------------------------------------------------------ AY231130 AGAAAAAGAAAAACAGAAAGAAAAAGAAAAACAAAAAGAGAAAGAAAAAGACGATGCTAG 9360 3 ------------------------------------------------------------ AY231130 TGACGGAAATGATGTGTCGACTAGCACAAGAACTGGAGAGAAAGATAGAGATGTCAATGT 9420
87 3 1 -------------------------CCGAGAATAAAATCATTTACTGACAAGATGATTTT 35 AY231130 CGGGACCAGTGGAACTTTCACGGTTCCGAGAATTAAATCATTCACTGATAAGATGATTCT 9480 ******** ******** ***** ********* * 9444 3 ACCGAAAATTAAAGGAAAAACTGTCCTTAATTTTAAATCATCTTCTTCAGTATAATCCGC 95 AY231130 ACCAAGAATTAAGGGAAAGACTGTCCTTAATTT-AAATCATCTTCTTCAGTATAATCCGC 9539 *** * ****** ***** ************** ************************** 3 AACAAATTGATATCTCAAACACTCGTGCCACTCAAACTCAATTCGAAAAGTGGTATGAGG 155 AY231130 AACAAATTGACATTTCTAACACTCGTGCCACTCAGTCACAATTTGAGAAATGGTATGAGG 9599 ********** ** ** ***************** * ***** ** ** ********** 3 GAGTGAGGAATGATTACGGTCTCAACGATAACGAAATGCAAGTG-ATGTTAAACGGTTTG 214 AY231130 GAGTGAGGAATGATTATGGTCTGAATGATAATGAAATGCAAGTG-ATGCTGAATGGCTTG 9658 **************** ***** ** ***** ************ *** * ** ** *** 3 ATGGTTTGGTGTATTGAAAATGGTACATCTCCAGACTTATCTGGTGTTTGGGTAATGATG 274 AY231130 ATGGTTTGGTGTATCGAGAATGGTACATCTCCAGACATATCTGGTGTTTGGGTTATGATG 9718 ************** ** ****************** **************** ****** 3 GATGGGGAAACACAAGTTGATTATCCCATTAAGCCTTTAATTGAACATGCAACTCCTTCG 334 AY231130 GATGGGGAAATTCAAGTTGACTATCCAATCAAGCCTTTAATTGAGCATGCTACCCCGTCA 9778 ********** ******** ***** ** ************** ***** ** ** ** 3 TTTAGGCAAATCATGGCTCACTTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGGAAT 394 AY231130 TTTAGGCAGATTATGGCTCACTTTAGTAACGCGGCAGAAGCATATATTGCAAAGAGAAAT 9838 ******** ** *********** ************** ***** ** ******** *** 3 395 G----------------------------------------------------------- AY2311309839 GCCACTGAGAGGTACATGCCGCGGTATGGAATCAAGAGAAATTTGACTGACATTAGCCTC 9898 * 3 ------------------------------------------------------------ AY231130 GCTAGATACGCTTTCGATTTCTATGAGGTTAATTCGAAAACACCTGATAGGGCTCGCGAA 3 ------------------------------------------------------------ AY231130 GCTCACATGCAGATGAAAGCTGCAGCGCTGCGAAACACTAGTCGCAGAATGTTTGGTATG 3 ------------------------------------------------------------ AY231130 GACGGCAGTGTTAGTAATAAGGAAGAAAACACGGAGAGACACACAGTGGAAGATGTCAAT 3 ------------------------------------------------------------ AY231130 AGAGACATGCACTCTCTCCTGGGTATGCGCAACTGAATACTCGCGCTGGTGTGTTTGTCG 3 ------------------------------------------------------------ AY231130 AGCTCGACTCGACCTTGTTTCACCTTATAGTACTAAATAAGCATTAGAATACAGTGTGGC 3 ------------------------------------------------------------ AY231130 TGTGCCACCACTTCTATTTTACAGTGAGGGTAGCCCTCCGTGCTTTTAGTATTATTCGAG 3 ------------------------------------------------------------ AY231130 TTCTCTGAGTCTCCATACAGTGTGGGTGGCCCACGTGTTATTTGAGCCTCTTAGAATGAG 3 -- AY231130 AG ALIGN 395 NU