Báo cáo khoa học: pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I
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Human kynurenine aminotransferase I/glutamine trans-aminase K (hKAT-I) is an important multifunctional enzyme. This study systematically studies the substrates of hKAT-I and reassesses the effects of pH, Tris, amino acids anda-keto acids on the activity of the enzyme. The experi-ments were comprised of functional expression of the hKAT-I in an insect cell/baculovirus expression system, purification of its recombinant protein, and functional characterization of the purified enzyme.
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